| http://purl.uniprot.org/citations/12609742 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12609742 | http://www.w3.org/2000/01/rdf-schema#comment | "The Wilms' tumor gene Wt1 encodes a zinc finger protein, which is required for normal formation of the genitourinary system and mesothelial tissues. Our recent findings indicate that Wt1 also plays a critical role in the development of ganglion cells in the vertebrate retina. Here we show that the POU-domain factor Pou4f2 (formerly Brn-3b), which is necessary for retinal ganglion cell survival, is up-regulated in human embryonic kidney (HEK)293 cells with stable Wt1 expression. Consistent with our previous observations of increased Pou4f2 mRNA in stably Wt1-transfeced HEK293 cells [EMBO J. 21 (2002) 1398], endogenous Pou4f2 was also elevated at the protein level in the HEK293 transfectants as well as in U2OS osteosarcoma cells that expressed an inducible Wt1 isoform. Transient co-transfection of a Wt1 expression construct activated a Pou4f2 promoter-reporter construct approximately 4-fold. Stimulation of the Pou4f2 promoter required a Wt1 binding element that was similar to a degenerative consensus site previously identified in other Wt1 responsive genes. Double-immunofluorescent labeling revealed co-expression of Pou4f2 and Wt1 in glomerular podocytes of adult kidney and in developing retinal ganglion cells of mouse embryos. Pou4f2 immunoreactivity was absent from the retinas of Wt1(-/-) embryos. In conclusion, we identified Pou4f2 as a novel downstream target gene of Wt1. Co-localization of both proteins in glomerular podocytes of the kidney and in developing retinal ganglion cells suggests a role for Wt1-Pou4f2 interaction in these tissues."xsd:string |
| http://purl.uniprot.org/citations/12609742 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0378-1119(02)01231-3"xsd:string |
| http://purl.uniprot.org/citations/12609742 | http://purl.uniprot.org/core/author | "Wagner N."xsd:string |
| http://purl.uniprot.org/citations/12609742 | http://purl.uniprot.org/core/author | "Scholz H."xsd:string |
| http://purl.uniprot.org/citations/12609742 | http://purl.uniprot.org/core/author | "Wagner K.D."xsd:string |
| http://purl.uniprot.org/citations/12609742 | http://purl.uniprot.org/core/author | "Schley G."xsd:string |
| http://purl.uniprot.org/citations/12609742 | http://purl.uniprot.org/core/author | "Theres H."xsd:string |
| http://purl.uniprot.org/citations/12609742 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12609742 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
| http://purl.uniprot.org/citations/12609742 | http://purl.uniprot.org/core/pages | "217-223"xsd:string |
| http://purl.uniprot.org/citations/12609742 | http://purl.uniprot.org/core/title | "The Wilms' tumor suppressor Wt1 encodes a transcriptional activator of the class IV POU-domain factor Pou4f2 (Brn-3b)."xsd:string |
| http://purl.uniprot.org/citations/12609742 | http://purl.uniprot.org/core/volume | "305"xsd:string |
| http://purl.uniprot.org/citations/12609742 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12609742 |
| http://purl.uniprot.org/citations/12609742 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12609742 |
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