| http://purl.uniprot.org/citations/12612062 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12612062 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12612062 | http://www.w3.org/2000/01/rdf-schema#comment | "The multisubunit transcription elongation factor NELF (for negative elongation factor) acts together with DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole) sensitivity-inducing factor (DSIF)/human Spt4-Spt5 to cause transcriptional pausing of RNA polymerase II (RNAPII). NELF activity is associated with five polypeptides, A to E. NELF-A has sequence similarity to hepatitis delta antigen (HDAg), the viral protein that binds to and activates RNAPII, whereas NELF-E is an RNA-binding protein whose RNA-binding activity is critical for NELF function. To understand the interactions of DSIF, NELF, and RNAPII at a molecular level, we identified the B, C, and D proteins of human NELF. NELF-B is identical to COBRA1, recently reported to associate with the product of breast cancer susceptibility gene BRCA1. NELF-C and NELF-D are highly related or identical to the protein called TH1, of unknown function. NELF-B and NELF-C or NELF-D are integral subunits that bring NELF-A and NELF-E together, and coexpression of these four proteins in insect cells resulted in the reconstitution of functionally active NELF. Detailed analyses using mutated recombinant complexes indicated that the small region of NELF-A with similarity to HDAg is critical for RNAPII binding and for transcriptional pausing. This study defines several important protein-protein interactions and opens the way for understanding the mechanism of DSIF- and NELF-induced transcriptional pausing."xsd:string |
| http://purl.uniprot.org/citations/12612062 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.23.6.1863-1873.2003"xsd:string |
| http://purl.uniprot.org/citations/12612062 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.23.6.1863-1873.2003"xsd:string |
| http://purl.uniprot.org/citations/12612062 | http://purl.uniprot.org/core/author | "Yano K."xsd:string |
| http://purl.uniprot.org/citations/12612062 | http://purl.uniprot.org/core/author | "Yano K."xsd:string |
| http://purl.uniprot.org/citations/12612062 | http://purl.uniprot.org/core/author | "Wada T."xsd:string |
| http://purl.uniprot.org/citations/12612062 | http://purl.uniprot.org/core/author | "Wada T."xsd:string |
| http://purl.uniprot.org/citations/12612062 | http://purl.uniprot.org/core/author | "Sugimoto S."xsd:string |
| http://purl.uniprot.org/citations/12612062 | http://purl.uniprot.org/core/author | "Sugimoto S."xsd:string |
| http://purl.uniprot.org/citations/12612062 | http://purl.uniprot.org/core/author | "Yamada T."xsd:string |
| http://purl.uniprot.org/citations/12612062 | http://purl.uniprot.org/core/author | "Yamada T."xsd:string |
| http://purl.uniprot.org/citations/12612062 | http://purl.uniprot.org/core/author | "Hasegawa J."xsd:string |
| http://purl.uniprot.org/citations/12612062 | http://purl.uniprot.org/core/author | "Hasegawa J."xsd:string |
| http://purl.uniprot.org/citations/12612062 | http://purl.uniprot.org/core/author | "Yamaguchi Y."xsd:string |
| http://purl.uniprot.org/citations/12612062 | http://purl.uniprot.org/core/author | "Yamaguchi Y."xsd:string |
| http://purl.uniprot.org/citations/12612062 | http://purl.uniprot.org/core/author | "Narita T."xsd:string |
| http://purl.uniprot.org/citations/12612062 | http://purl.uniprot.org/core/author | "Narita T."xsd:string |
| http://purl.uniprot.org/citations/12612062 | http://purl.uniprot.org/core/author | "Kim D.-K."xsd:string |
| http://purl.uniprot.org/citations/12612062 | http://purl.uniprot.org/core/author | "Kim D.-K."xsd:string |
| http://purl.uniprot.org/citations/12612062 | http://purl.uniprot.org/core/author | "Handa H."xsd:string |
| http://purl.uniprot.org/citations/12612062 | http://purl.uniprot.org/core/author | "Handa H."xsd:string |
| http://purl.uniprot.org/citations/12612062 | http://purl.uniprot.org/core/author | "Endoh M."xsd:string |
| http://purl.uniprot.org/citations/12612062 | http://purl.uniprot.org/core/author | "Endoh M."xsd:string |