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http://purl.uniprot.org/citations/12625842http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/12625842http://www.w3.org/2000/01/rdf-schema#comment"

Background

Pigment epithelium-derived factor (PEDF) has binding affinity for cell-surface receptors in retinoblastoma cells and for glycosaminoglycans. We investigated the effects of glycosaminoglycans on PEDF-receptor interactions.

Results

125I-PEDF formed complexes with protease-resistant components of medium conditioned by human retinoblastoma Y-79 cells. Using specific glycosaminoglycan degrading enzymes in spectrophotometric assays and PEDF-affinity chromatography, we detected heparin and heparan sulfate-like glycosaminoglycans in the Y-79 conditioned media, which had binding affinity for PEDF. The Y-79 conditioned media significantly enhanced the binding of 125I-PEDF to Y-79 cell-surface receptors. However, enzymatic and chemical depletion of sulfated glycosaminoglycans from the Y-79 cell cultures by heparitinase and chlorate treatments decreased the degree of 125I-PEDF binding to cell-surface receptors.

Conclusions

These data indicate that retinoblastoma cells secrete heparin/heparan sulfate with binding affinity for PEDF, which may be important in efficient cell-surface receptor binding."xsd:string
http://purl.uniprot.org/citations/12625842http://purl.org/dc/terms/identifier"doi:10.1186/1471-2091-4-1"xsd:string
http://purl.uniprot.org/citations/12625842http://purl.uniprot.org/core/author"Becerra S.P."xsd:string
http://purl.uniprot.org/citations/12625842http://purl.uniprot.org/core/author"Weldon J.E."xsd:string
http://purl.uniprot.org/citations/12625842http://purl.uniprot.org/core/author"Alberdi E.M."xsd:string
http://purl.uniprot.org/citations/12625842http://purl.uniprot.org/core/date"2003"xsd:gYear
http://purl.uniprot.org/citations/12625842http://purl.uniprot.org/core/name"BMC Biochem"xsd:string
http://purl.uniprot.org/citations/12625842http://purl.uniprot.org/core/pages"1"xsd:string
http://purl.uniprot.org/citations/12625842http://purl.uniprot.org/core/title"Glycosaminoglycans in human retinoblastoma cells: heparan sulfate, a modulator of the pigment epithelium-derived factor-receptor interactions."xsd:string
http://purl.uniprot.org/citations/12625842http://purl.uniprot.org/core/volume"4"xsd:string
http://purl.uniprot.org/citations/12625842http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/12625842
http://purl.uniprot.org/citations/12625842http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/12625842
http://purl.uniprot.org/uniprot/#_O67322-mappedCitation-12625842http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/12625842
http://purl.uniprot.org/uniprot/O67322http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/12625842