| http://purl.uniprot.org/citations/12627947 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12627947 | http://www.w3.org/2000/01/rdf-schema#comment | "A new ruthenium-cytochrome c derivative was designed to study electron transfer from cytochrome bc1 to cytochrome c (Cc). The single sulfhydryl on yeast H39C;C102T iso-1-Cc was labeled with Ru(2,2'-bipyrazine)2(4-bromomethyl-4'-methyl-2,2'-bipyridine) to form Ru(z)-39-Cc. The Ru(z)-39-Cc derivative has the same steady-state activity with yeast cytochrome bc1 as wild-type yeast iso-1-Cc, indicating that the ruthenium complex does not interfere in the binding interaction. Laser excitation of reduced Ru(z)-39-Cc results in electron transfer from heme c to the excited state of ruthenium with a rate constant of 1.5 x 10(6) x s(-1). The resulting Ru(I) is rapidly oxidized by atmospheric oxygen in the buffer. The yield of photooxidized heme c is 20% in a single flash. Flash photolysis of a 1:1 complex between reduced yeast cytochrome bc1 and Ru(z)-39-Cc at low ionic strength leads to rapid photooxidation of heme c, followed by intracomplex electron transfer from cytochrome c1 to heme c with a rate constant of 1.4 x 10(4) x s(-1). As the ionic strength is raised above 100 mM, the intracomplex phase disappears, and a new phase appears due to the bimolecular reaction between solution Ru-39-Cc and cytochrome bc1. The interaction of yeast Ru-39-Cc with yeast cytochrome bc1 is stronger than that of horse Ru-39-Cc with bovine cytochrome bc1, suggesting that nonpolar interactions are stronger in the yeast system."xsd:string |
| http://purl.uniprot.org/citations/12627947 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi027213g"xsd:string |
| http://purl.uniprot.org/citations/12627947 | http://purl.uniprot.org/core/author | "Yu L."xsd:string |
| http://purl.uniprot.org/citations/12627947 | http://purl.uniprot.org/core/author | "Xiao K."xsd:string |
| http://purl.uniprot.org/citations/12627947 | http://purl.uniprot.org/core/author | "Yu C.A."xsd:string |
| http://purl.uniprot.org/citations/12627947 | http://purl.uniprot.org/core/author | "Pielak G.J."xsd:string |
| http://purl.uniprot.org/citations/12627947 | http://purl.uniprot.org/core/author | "Rajagukguk S."xsd:string |
| http://purl.uniprot.org/citations/12627947 | http://purl.uniprot.org/core/author | "Trumpower B."xsd:string |
| http://purl.uniprot.org/citations/12627947 | http://purl.uniprot.org/core/author | "Engstrom G."xsd:string |
| http://purl.uniprot.org/citations/12627947 | http://purl.uniprot.org/core/author | "Patel C.N."xsd:string |
| http://purl.uniprot.org/citations/12627947 | http://purl.uniprot.org/core/author | "Saunders A.J."xsd:string |
| http://purl.uniprot.org/citations/12627947 | http://purl.uniprot.org/core/author | "Merbitz-Zahradnik T."xsd:string |
| http://purl.uniprot.org/citations/12627947 | http://purl.uniprot.org/core/author | "Millett F."xsd:string |
| http://purl.uniprot.org/citations/12627947 | http://purl.uniprot.org/core/author | "Durham B."xsd:string |
| http://purl.uniprot.org/citations/12627947 | http://purl.uniprot.org/core/author | "Rajagukguk R."xsd:string |
| http://purl.uniprot.org/citations/12627947 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12627947 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/12627947 | http://purl.uniprot.org/core/pages | "2816-2824"xsd:string |
| http://purl.uniprot.org/citations/12627947 | http://purl.uniprot.org/core/title | "Design of a ruthenium-labeled cytochrome c derivative to study electron transfer with the cytochrome bc1 complex."xsd:string |
| http://purl.uniprot.org/citations/12627947 | http://purl.uniprot.org/core/volume | "42"xsd:string |
| http://purl.uniprot.org/citations/12627947 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12627947 |
| http://purl.uniprot.org/citations/12627947 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12627947 |
| http://purl.uniprot.org/uniprot/#_P08067-mappedCitation-12627947 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12627947 |
| http://purl.uniprot.org/uniprot/#_P08525-mappedCitation-12627947 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12627947 |