| http://purl.uniprot.org/citations/12628934 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12628934 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12628934 | http://www.w3.org/2000/01/rdf-schema#comment | "We have examined the genetic requirements for efficient repair of a site-specific DNA double-strand break (DSB) in Schizosaccharomyces pombe. Tech nology was developed in which a unique DSB could be generated in a non-essential minichromosome, Ch(16), using the Saccharomyces cerevisiae HO-endonuclease and its target site, MATa. DSB repair in this context was predominantly through interchromosomal gene conversion. We found that the homologous recombination (HR) genes rhp51(+), rad22A(+), rad32(+) and the nucleotide excision repair gene rad16(+) were required for efficient interchromosomal gene conversion. Further, DSB-induced cell cycle delay and efficient HR required the DNA integrity checkpoint gene rad3(+). Rhp55 was required for interchromosomal gene conversion; however, an alternative DSB repair mechanism was used in an rhp55Delta background involving ku70(+) and rhp51(+). Surprisingly, DSB-induced minichromosome loss was significantly reduced in ku70Delta and lig4Delta non-homologous end joining (NHEJ) mutant backgrounds compared with wild type. Furthermore, roles for Ku70 and Lig4 were identified in suppressing DSB-induced chromosomal rearrangements associated with gene conversion. These findings are consistent with both competitive and cooperative interactions between components of the HR and NHEJ pathways."xsd:string |
| http://purl.uniprot.org/citations/12628934 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/cdg119"xsd:string |
| http://purl.uniprot.org/citations/12628934 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/cdg119"xsd:string |
| http://purl.uniprot.org/citations/12628934 | http://purl.uniprot.org/core/author | "Humphrey T."xsd:string |
| http://purl.uniprot.org/citations/12628934 | http://purl.uniprot.org/core/author | "Humphrey T."xsd:string |
| http://purl.uniprot.org/citations/12628934 | http://purl.uniprot.org/core/author | "Thacker J."xsd:string |
| http://purl.uniprot.org/citations/12628934 | http://purl.uniprot.org/core/author | "Thacker J."xsd:string |
| http://purl.uniprot.org/citations/12628934 | http://purl.uniprot.org/core/author | "Prudden J."xsd:string |
| http://purl.uniprot.org/citations/12628934 | http://purl.uniprot.org/core/author | "Prudden J."xsd:string |
| http://purl.uniprot.org/citations/12628934 | http://purl.uniprot.org/core/author | "Deans B."xsd:string |
| http://purl.uniprot.org/citations/12628934 | http://purl.uniprot.org/core/author | "Deans B."xsd:string |
| http://purl.uniprot.org/citations/12628934 | http://purl.uniprot.org/core/author | "Evans J.S."xsd:string |
| http://purl.uniprot.org/citations/12628934 | http://purl.uniprot.org/core/author | "Evans J.S."xsd:string |
| http://purl.uniprot.org/citations/12628934 | http://purl.uniprot.org/core/author | "Hussey S.P."xsd:string |
| http://purl.uniprot.org/citations/12628934 | http://purl.uniprot.org/core/author | "Hussey S.P."xsd:string |
| http://purl.uniprot.org/citations/12628934 | http://purl.uniprot.org/core/author | "O'Neill P."xsd:string |
| http://purl.uniprot.org/citations/12628934 | http://purl.uniprot.org/core/author | "O'Neill P."xsd:string |
| http://purl.uniprot.org/citations/12628934 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12628934 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12628934 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/12628934 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/12628934 | http://purl.uniprot.org/core/pages | "1419-1430"xsd:string |
| http://purl.uniprot.org/citations/12628934 | http://purl.uniprot.org/core/pages | "1419-1430"xsd:string |