| http://purl.uniprot.org/citations/12630574 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12630574 | http://www.w3.org/2000/01/rdf-schema#comment | "It has been shown that human airway trypsin-like protease (HAT) is localized in human bronchial epithelial cells (HBEC), and trypsin activates protease-activated receptor-2 (PAR-2). Activation of PAR-2 activates G-protein followed by an increase of intracellular free Ca2+, [Ca2+]in. This study was undertaken to clarify whether HAT can activate PAR-2 in HBEC or not. RT-PCR showed that HAT mRNA is expressed in HBEC, and PAR-2 mRNA is the most strongly expressed of the known PARs in HBEC. Both PAR-2 agonist peptide (PAR-2 AP) and HAT increased [Ca2+]in in HBEC in a biphasic fashion; a prompt, sharp increase (peak I) and a sustained low plateau (peak II). PAR-2 AP over 100-200 microM and HAT over 200-300 mU/ml (0.08-0.12 microM) induced both peak I and II, and PAR-2 AP below 100 microM and HAT below 200 mU/ml induced only peak II. Both PAR-2 AP-induced and HAT-induced peak I were induced by Ca2+ mobilization from intracellular stores, because they appeared even in Ca2+-free medium. Both PAR-2 AP-induced and HAT-induced peak II were induced by an influx of extracellular Ca2+, because they were abolished in Ca2+-free medium. The Ca2+ response to HAT was desensitized by exposure of HBEC to PAR-2 AP. These results indicate that HBEC have a functional PAR-2, and HAT regulates cellular functions of HBEC via activation of PAR-2."xsd:string |
| http://purl.uniprot.org/citations/12630574 | http://purl.uniprot.org/core/author | "Nakamura Y."xsd:string |
| http://purl.uniprot.org/citations/12630574 | http://purl.uniprot.org/core/author | "Yoneda K."xsd:string |
| http://purl.uniprot.org/citations/12630574 | http://purl.uniprot.org/core/author | "Takahashi A."xsd:string |
| http://purl.uniprot.org/citations/12630574 | http://purl.uniprot.org/core/author | "Miki M."xsd:string |
| http://purl.uniprot.org/citations/12630574 | http://purl.uniprot.org/core/author | "Eguchi H."xsd:string |
| http://purl.uniprot.org/citations/12630574 | http://purl.uniprot.org/core/author | "Nakaya Y."xsd:string |
| http://purl.uniprot.org/citations/12630574 | http://purl.uniprot.org/core/author | "Sone S."xsd:string |
| http://purl.uniprot.org/citations/12630574 | http://purl.uniprot.org/core/author | "Yasuoka S."xsd:string |
| http://purl.uniprot.org/citations/12630574 | http://purl.uniprot.org/core/author | "Masegi T."xsd:string |
| http://purl.uniprot.org/citations/12630574 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12630574 | http://purl.uniprot.org/core/name | "J Med Invest"xsd:string |
| http://purl.uniprot.org/citations/12630574 | http://purl.uniprot.org/core/pages | "95-107"xsd:string |
| http://purl.uniprot.org/citations/12630574 | http://purl.uniprot.org/core/title | "Effect of human airway trypsin-like protease on intracellular free Ca2+ concentration in human bronchial epithelial cells."xsd:string |
| http://purl.uniprot.org/citations/12630574 | http://purl.uniprot.org/core/volume | "50"xsd:string |
| http://purl.uniprot.org/citations/12630574 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12630574 |
| http://purl.uniprot.org/citations/12630574 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12630574 |
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| http://purl.uniprot.org/uniprot/#_B4DL57-mappedCitation-12630574 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12630574 |
| http://purl.uniprot.org/uniprot/#_P55085-mappedCitation-12630574 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12630574 |
| http://purl.uniprot.org/uniprot/#_O60235-mappedCitation-12630574 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12630574 |
| http://purl.uniprot.org/uniprot/#_Q4W5K4-mappedCitation-12630574 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12630574 |
| http://purl.uniprot.org/uniprot/O60235 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12630574 |
| http://purl.uniprot.org/uniprot/B4DL57 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12630574 |