| http://purl.uniprot.org/citations/12637569 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12637569 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12637569 | http://www.w3.org/2000/01/rdf-schema#comment | "Bone morphogenetic protein (BMP)-1 is a zinc-dependent metalloproteinase that cleaves a variety of extracellular matrix substrates, including type I procollagen. Little is known about the site of action of BMP-1, although the extracellular matrix seems likely to be it. BMP-1 is synthesized with an N-terminal prodomain. The removal of the prodomain presumably activates the proteinase. In this study we show that the prodomain is cleaved in the trans-Golgi network (TGN) and by furin-like/paired basic proprotein convertases. Inhibitors of furin resulted in the secretion of pro-BMP-1, which could not cleave procollagen. Recombinant furin cleaved the prodomain from pro-BMP-1. Site-directed mutagenesis of the prodomain cleavage site (RSRR) to RSAA resulted in efficient secretion of pro-BMP-1. Therefore, prodomain cleavage was not required for secretion. Using peptide N-glycosidase and neuraminidase digestion to determine the post-translational status of pro-BMP-1 during its conversion to BMP-1, we showed that BMP-1 first appears in the TGN during sialylation of the molecule. Furthermore, immunofluorescence studies using an antibody to the nascent N terminus of BMP-1 showed localization to the TGN and plasma membrane. The observation that BMP-1 occurs inside the cell raises the possibility that BMP-1 might begin to cleave its substrates prior to secretion to the extracellular matrix."xsd:string |
| http://purl.uniprot.org/citations/12637569 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m213021200"xsd:string |
| http://purl.uniprot.org/citations/12637569 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m213021200"xsd:string |
| http://purl.uniprot.org/citations/12637569 | http://purl.uniprot.org/core/author | "Kadler K.E."xsd:string |
| http://purl.uniprot.org/citations/12637569 | http://purl.uniprot.org/core/author | "Kadler K.E."xsd:string |
| http://purl.uniprot.org/citations/12637569 | http://purl.uniprot.org/core/author | "Leighton M."xsd:string |
| http://purl.uniprot.org/citations/12637569 | http://purl.uniprot.org/core/author | "Leighton M."xsd:string |
| http://purl.uniprot.org/citations/12637569 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12637569 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12637569 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12637569 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12637569 | http://purl.uniprot.org/core/pages | "18478-18484"xsd:string |
| http://purl.uniprot.org/citations/12637569 | http://purl.uniprot.org/core/pages | "18478-18484"xsd:string |
| http://purl.uniprot.org/citations/12637569 | http://purl.uniprot.org/core/title | "Paired basic/Furin-like proprotein convertase cleavage of Pro-BMP-1 in the trans-Golgi network."xsd:string |
| http://purl.uniprot.org/citations/12637569 | http://purl.uniprot.org/core/title | "Paired basic/Furin-like proprotein convertase cleavage of Pro-BMP-1 in the trans-Golgi network."xsd:string |
| http://purl.uniprot.org/citations/12637569 | http://purl.uniprot.org/core/volume | "278"xsd:string |
| http://purl.uniprot.org/citations/12637569 | http://purl.uniprot.org/core/volume | "278"xsd:string |
| http://purl.uniprot.org/citations/12637569 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12637569 |
| http://purl.uniprot.org/citations/12637569 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12637569 |
| http://purl.uniprot.org/citations/12637569 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12637569 |
| http://purl.uniprot.org/citations/12637569 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12637569 |
| http://purl.uniprot.org/uniprot/P13497 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/12637569 |
| http://purl.uniprot.org/uniprot/P13497#attribution-6962FC8F702DD8B9B9E032AAB183DB12 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/12637569 |