| http://purl.uniprot.org/citations/12642575 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12642575 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12642575 | http://www.w3.org/2000/01/rdf-schema#comment | "Tyvelose epimerase catalyzes the last step in the biosynthesis of tyvelose by converting CDP-d-paratose to CDP-d-tyvelose. This unusual 3,6-dideoxyhexose occurs in the O-antigens of some types of Gram-negative bacteria. Here we describe the cloning, protein purification, and high-resolution x-ray crystallographic analysis of tyvelose epimerase from Salmonella typhi complexed with CDP. The enzyme from S. typhi is a homotetramer with each subunit containing 339 amino acid residues and a tightly bound NAD+ cofactor. The quaternary structure of the enzyme displays 222 symmetry and can be aptly described as a dimer of dimers. Each subunit folds into two distinct lobes: the N-terminal motif responsible for NAD+ binding and the C-terminal region that harbors the binding site for CDP. The analysis described here demonstrates that tyvelose epimerase belongs to the short-chain dehydrogenase/reductase superfamily of enzymes. Indeed, its active site is reminiscent to that observed for UDP-galactose 4-epimerase, an enzyme that plays a key role in galactose metabolism. Unlike UDP-galactose 4-epimerase where the conversion of configuration occurs about C-4 of the UDP-glucose or UDP-galactose substrates, in the reaction catalyzed by tyvelose epimerase, the inversion of stereochemistry occurs at C-2. On the basis of the observed binding mode for CDP, it is possible to predict the manner in which the substrate, CDP-paratose, and the product, CDP-tyvelose, might be accommodated within the active site of tyvelose epimerase."xsd:string |
| http://purl.uniprot.org/citations/12642575 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m301948200"xsd:string |
| http://purl.uniprot.org/citations/12642575 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m301948200"xsd:string |
| http://purl.uniprot.org/citations/12642575 | http://purl.uniprot.org/core/author | "Holden H.M."xsd:string |
| http://purl.uniprot.org/citations/12642575 | http://purl.uniprot.org/core/author | "Holden H.M."xsd:string |
| http://purl.uniprot.org/citations/12642575 | http://purl.uniprot.org/core/author | "Koropatkin N.M."xsd:string |
| http://purl.uniprot.org/citations/12642575 | http://purl.uniprot.org/core/author | "Koropatkin N.M."xsd:string |
| http://purl.uniprot.org/citations/12642575 | http://purl.uniprot.org/core/author | "Liu H.-W."xsd:string |
| http://purl.uniprot.org/citations/12642575 | http://purl.uniprot.org/core/author | "Liu H.-W."xsd:string |
| http://purl.uniprot.org/citations/12642575 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12642575 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12642575 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12642575 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12642575 | http://purl.uniprot.org/core/pages | "20874-20881"xsd:string |
| http://purl.uniprot.org/citations/12642575 | http://purl.uniprot.org/core/pages | "20874-20881"xsd:string |
| http://purl.uniprot.org/citations/12642575 | http://purl.uniprot.org/core/title | "High resolution X-ray structure of tyvelose epimerase from Salmonella typhi."xsd:string |
| http://purl.uniprot.org/citations/12642575 | http://purl.uniprot.org/core/title | "High resolution X-ray structure of tyvelose epimerase from Salmonella typhi."xsd:string |
| http://purl.uniprot.org/citations/12642575 | http://purl.uniprot.org/core/volume | "278"xsd:string |
| http://purl.uniprot.org/citations/12642575 | http://purl.uniprot.org/core/volume | "278"xsd:string |
| http://purl.uniprot.org/citations/12642575 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12642575 |
| http://purl.uniprot.org/citations/12642575 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12642575 |
| http://purl.uniprot.org/citations/12642575 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12642575 |
| http://purl.uniprot.org/citations/12642575 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12642575 |