| http://purl.uniprot.org/citations/12646217 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12646217 | http://www.w3.org/2000/01/rdf-schema#comment | "The Src-homology 2 domain containing protein tyrosine phosphatase-1 (SHP-1) is involved in the pathogenesis of infection with Leishmania. Recently, we identified elongation factor-1 alpha (EF-1 alpha) from Leishmania donovani as a SHP-1 binding and activating protein [J. Biol. Chem. 277 (2002) 50190]. To characterize this apparent Leishmania virulence factor further, the cDNA encoding L. donovani EF-1 alpha was cloned and sequenced. Whereas nearly complete sequence conservation was observed amongst EF-1 alpha proteins from trypanosomatids, the deduced amino acid sequence of EF-1 alpha of L. donovani when compared to mammalian EF-1 alpha sequences showed a number of significant changes. Protein structure modeling-based upon the known crystal structure of EF-1 alpha for Saccharomyces cerevisiae-identified a hairpin loop present in mammalian EF-1 alpha and absent from the Leishmania protein which corresponded to a 12 amino acid deletion. Consistent with these structural differences, the sub-cellular distributions of L. donovani EF-1 alpha and host EF-1 alpha were strikingly different. Interestingly, infection of macrophages with L. donovani caused redistribution of host as well as pathogen EF-1 alpha. Since EF-1 alpha is essential for survival, the distinct biochemical and structural properties of Leishmania EF-1 alpha may provide a novel target for drug development."xsd:string |
| http://purl.uniprot.org/citations/12646217 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0006-291x(03)00216-x"xsd:string |
| http://purl.uniprot.org/citations/12646217 | http://purl.uniprot.org/core/author | "Yi T."xsd:string |
| http://purl.uniprot.org/citations/12646217 | http://purl.uniprot.org/core/author | "Reiner N.E."xsd:string |
| http://purl.uniprot.org/citations/12646217 | http://purl.uniprot.org/core/author | "Cherkasov A."xsd:string |
| http://purl.uniprot.org/citations/12646217 | http://purl.uniprot.org/core/author | "Nandan D."xsd:string |
| http://purl.uniprot.org/citations/12646217 | http://purl.uniprot.org/core/author | "Sabouti R."xsd:string |
| http://purl.uniprot.org/citations/12646217 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12646217 | http://purl.uniprot.org/core/name | "Biochem Biophys Res Commun"xsd:string |
| http://purl.uniprot.org/citations/12646217 | http://purl.uniprot.org/core/pages | "646-652"xsd:string |
| http://purl.uniprot.org/citations/12646217 | http://purl.uniprot.org/core/title | "Molecular cloning, biochemical and structural analysis of elongation factor-1 alpha from Leishmania donovani: comparison with the mammalian homologue."xsd:string |
| http://purl.uniprot.org/citations/12646217 | http://purl.uniprot.org/core/volume | "302"xsd:string |
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