| http://purl.uniprot.org/citations/12665572 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12665572 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12665572 | http://www.w3.org/2000/01/rdf-schema#comment | "Embryonic stem (ES) cells are immortal and pluripotent cells derived from early mammalian embryos. Transcription factor Oct3/4 is essential for self-renewal of ES cells and early mouse development. However, only a few Oct3/4 target genes have been identified. In this study, we found that F-box-containing protein Fbx15 was expressed predominantly in mouse undifferentiated ES cells. Inactivation of Oct3/4 in ES cells led to rapid extinction of Fbx15 expression. Reporter gene analyses demonstrated that this ES cell-specific expression required an 18-bp enhancer element located approximately 500 nucleotides upstream from the transcription initiation site. The enhancer contained an octamer-like motif and an adjacent Sox-binding motif. Deletion or point mutation of either motif abolished the enhancer activity. The 18-bp fragment became active in NIH 3T3 cells when Oct3/4 and Sox2 were coexpressed. A gel mobility shift assay demonstrated cooperative binding of Oct3/4 and Sox2 to the enhancer sequence. In mice having a beta-galactosidase gene knocked into the Fbx15 locus, 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside staining was detected in ES cells, early embryos (two-cell to blastocyst stages), and testis tissue. Despite such specific expression of Fbx15, homozygous mutant mice showed no gross developmental defects and were fertile. Fbx15-null ES cells were normal in morphology, proliferation, and differentiation. These data demonstrate that Fbx15 is a novel target of Oct3/4 but is dispensable for ES cell self-renewal, development, and fertility."xsd:string |
| http://purl.uniprot.org/citations/12665572 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.23.8.2699-2708.2003"xsd:string |
| http://purl.uniprot.org/citations/12665572 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.23.8.2699-2708.2003"xsd:string |
| http://purl.uniprot.org/citations/12665572 | http://purl.uniprot.org/core/author | "Takahashi K."xsd:string |
| http://purl.uniprot.org/citations/12665572 | http://purl.uniprot.org/core/author | "Takahashi K."xsd:string |
| http://purl.uniprot.org/citations/12665572 | http://purl.uniprot.org/core/author | "Maeda M."xsd:string |
| http://purl.uniprot.org/citations/12665572 | http://purl.uniprot.org/core/author | "Maeda M."xsd:string |
| http://purl.uniprot.org/citations/12665572 | http://purl.uniprot.org/core/author | "Niwa H."xsd:string |
| http://purl.uniprot.org/citations/12665572 | http://purl.uniprot.org/core/author | "Niwa H."xsd:string |
| http://purl.uniprot.org/citations/12665572 | http://purl.uniprot.org/core/author | "Maruyama M."xsd:string |
| http://purl.uniprot.org/citations/12665572 | http://purl.uniprot.org/core/author | "Maruyama M."xsd:string |
| http://purl.uniprot.org/citations/12665572 | http://purl.uniprot.org/core/author | "Yamanaka S."xsd:string |
| http://purl.uniprot.org/citations/12665572 | http://purl.uniprot.org/core/author | "Yamanaka S."xsd:string |
| http://purl.uniprot.org/citations/12665572 | http://purl.uniprot.org/core/author | "Tokuzawa Y."xsd:string |
| http://purl.uniprot.org/citations/12665572 | http://purl.uniprot.org/core/author | "Tokuzawa Y."xsd:string |
| http://purl.uniprot.org/citations/12665572 | http://purl.uniprot.org/core/author | "Mitsui K."xsd:string |
| http://purl.uniprot.org/citations/12665572 | http://purl.uniprot.org/core/author | "Mitsui K."xsd:string |
| http://purl.uniprot.org/citations/12665572 | http://purl.uniprot.org/core/author | "Kaiho E."xsd:string |
| http://purl.uniprot.org/citations/12665572 | http://purl.uniprot.org/core/author | "Kaiho E."xsd:string |
| http://purl.uniprot.org/citations/12665572 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12665572 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12665572 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/12665572 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |