| http://purl.uniprot.org/citations/12672804 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12672804 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12672804 | http://www.w3.org/2000/01/rdf-schema#comment | "Tumor immunology has received a large impetus from the identification of tumor-associated antigens. Among them, a monoclonal antibody, 22.1.1, was instrumental in defining a novel tumor-associated antigen that was termed "receptor binding cancer antigen expressed on SiSo cells" (RCAS1). RCAS1 was proposed to induce growth arrest and apoptosis on activated immune cells, mediated by a putative death receptor. Structurally, RCAS1 was predicted to exist as a type II transmembrane protein and in a soluble form. Here, we analyzed occurrence, membrane topology, and subcellular localization of the RCAS1-encoded gene product. RCAS1 was shown to be a ubiquitously expressed type III transmembrane protein with a Golgi-predominant localization. Monoclonal antibody 22.1.1 failed to recognize RCAS1, as demonstrated by confocal microscopy. Instead, we showed that the cognate 22.1.1 epitope is identical with the tumor-associated O-linked glycan Tn (N-acetyl-d-galactosamine, GalNAc). Overexpression of RCAS1 in cell lines that are negative for 22.1.1 surface staining led to the generation of Tn and the closely related TF (Thomsen-Friedenreich, Galbeta1-3GalNAc) antigen, thus providing a functional link to the generation of the 22.1.1 epitope. We suggest that RCAS1 modulates surface expression of tumor-associated, normally cryptic O-linked glycan structures and contributes indirectly to the antigenicity of tumor cells."xsd:string |
| http://purl.uniprot.org/citations/12672804 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m301361200"xsd:string |
| http://purl.uniprot.org/citations/12672804 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m301361200"xsd:string |
| http://purl.uniprot.org/citations/12672804 | http://purl.uniprot.org/core/author | "Rehm A."xsd:string |
| http://purl.uniprot.org/citations/12672804 | http://purl.uniprot.org/core/author | "Rehm A."xsd:string |
| http://purl.uniprot.org/citations/12672804 | http://purl.uniprot.org/core/author | "Engelsberg A."xsd:string |
| http://purl.uniprot.org/citations/12672804 | http://purl.uniprot.org/core/author | "Engelsberg A."xsd:string |
| http://purl.uniprot.org/citations/12672804 | http://purl.uniprot.org/core/author | "Schuelein R."xsd:string |
| http://purl.uniprot.org/citations/12672804 | http://purl.uniprot.org/core/author | "Schuelein R."xsd:string |
| http://purl.uniprot.org/citations/12672804 | http://purl.uniprot.org/core/author | "Karsten U."xsd:string |
| http://purl.uniprot.org/citations/12672804 | http://purl.uniprot.org/core/author | "Karsten U."xsd:string |
| http://purl.uniprot.org/citations/12672804 | http://purl.uniprot.org/core/author | "Hermosilla R."xsd:string |
| http://purl.uniprot.org/citations/12672804 | http://purl.uniprot.org/core/author | "Hermosilla R."xsd:string |
| http://purl.uniprot.org/citations/12672804 | http://purl.uniprot.org/core/author | "Doerken B."xsd:string |
| http://purl.uniprot.org/citations/12672804 | http://purl.uniprot.org/core/author | "Doerken B."xsd:string |
| http://purl.uniprot.org/citations/12672804 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12672804 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12672804 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12672804 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12672804 | http://purl.uniprot.org/core/pages | "22998-23007"xsd:string |
| http://purl.uniprot.org/citations/12672804 | http://purl.uniprot.org/core/pages | "22998-23007"xsd:string |
| http://purl.uniprot.org/citations/12672804 | http://purl.uniprot.org/core/title | "The Golgi protein RCAS1 controls cell surface expression of tumor-associated O-linked glycan antigens."xsd:string |
| http://purl.uniprot.org/citations/12672804 | http://purl.uniprot.org/core/title | "The Golgi protein RCAS1 controls cell surface expression of tumor-associated O-linked glycan antigens."xsd:string |