| http://purl.uniprot.org/citations/12676694 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12676694 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12676694 | http://www.w3.org/2000/01/rdf-schema#comment | "In a previous study we isolated the meta-cleavage enzyme gene, tesB, that encodes an enzyme that carries out a meta-cleavage reaction in the breakdown of testosterone by Comamonas testeroni TA441 (M. Horinouchi et al., Microbiology 147:3367-3375, 2001). Here we report the isolation of a gene, tesD, that encodes a hydrolase which acts on the product of the meta-cleavage reaction. We isolated tesD by using a Tn5 mutant of TA441 that showed limited growth on testosterone. TesD exhibited ca. 40% identity in amino acid sequence with BphDs, known hydrolases of biphenyl degradation in Pseudomonas spp. The TesD-disrupted mutant showed limited growth on testosterone, and the culture shows an intense yellow color. High-pressure liquid chromatography analysis of the culture of TesD-disrupted mutant incubated with testosterone detected five major intermediate compounds, one of which, showing yellow color under neutral conditions, was considered to be the product of the meta-cleavage reaction. The methylation product was analyzed and identified as methyl-4,5-9,10-diseco-3-methoxy-5,9,17-trioxoandrosta-1(10),2-dien-4-oate, indicating that the substrate of TesD in testosterone degradation is 4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-dien-4-oic acid. 4,5-9,10-Diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-dien-4-oic acid was transformed by Escherichia coli-expressed TesD. Downstream of tesD, we identified tesE, F, and G, which encode for enzymes that degrade one of the products of 4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-dien-4-oic acid converted by TesD."xsd:string |
| http://purl.uniprot.org/citations/12676694 | http://purl.org/dc/terms/identifier | "doi:10.1128/aem.69.4.2139-2152.2003"xsd:string |
| http://purl.uniprot.org/citations/12676694 | http://purl.org/dc/terms/identifier | "doi:10.1128/aem.69.4.2139-2152.2003"xsd:string |
| http://purl.uniprot.org/citations/12676694 | http://purl.org/dc/terms/identifier | "doi:10.1128/AEM.69.4.2139-2152.2003"xsd:string |
| http://purl.uniprot.org/citations/12676694 | http://purl.uniprot.org/core/author | "Hayashi T."xsd:string |
| http://purl.uniprot.org/citations/12676694 | http://purl.uniprot.org/core/author | "Hayashi T."xsd:string |
| http://purl.uniprot.org/citations/12676694 | http://purl.uniprot.org/core/author | "Horinouchi M."xsd:string |
| http://purl.uniprot.org/citations/12676694 | http://purl.uniprot.org/core/author | "Horinouchi M."xsd:string |
| http://purl.uniprot.org/citations/12676694 | http://purl.uniprot.org/core/author | "Koshino H."xsd:string |
| http://purl.uniprot.org/citations/12676694 | http://purl.uniprot.org/core/author | "Koshino H."xsd:string |
| http://purl.uniprot.org/citations/12676694 | http://purl.uniprot.org/core/author | "Kudo T."xsd:string |
| http://purl.uniprot.org/citations/12676694 | http://purl.uniprot.org/core/author | "Kudo T."xsd:string |
| http://purl.uniprot.org/citations/12676694 | http://purl.uniprot.org/core/author | "Yamamoto T."xsd:string |
| http://purl.uniprot.org/citations/12676694 | http://purl.uniprot.org/core/author | "Yamamoto T."xsd:string |
| http://purl.uniprot.org/citations/12676694 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12676694 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12676694 | http://purl.uniprot.org/core/name | "Appl. Environ. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/12676694 | http://purl.uniprot.org/core/name | "Appl. Environ. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/12676694 | http://purl.uniprot.org/core/pages | "2139-2152"xsd:string |
| http://purl.uniprot.org/citations/12676694 | http://purl.uniprot.org/core/pages | "2139-2152"xsd:string |
| http://purl.uniprot.org/citations/12676694 | http://purl.uniprot.org/core/title | "Gene encoding the hydrolase for the product of the meta-cleavage reaction in testosterone degradation by Comamonas testosteroni."xsd:string |
| http://purl.uniprot.org/citations/12676694 | http://purl.uniprot.org/core/title | "Gene encoding the hydrolase for the product of the meta-cleavage reaction in testosterone degradation by Comamonas testosteroni."xsd:string |
| http://purl.uniprot.org/citations/12676694 | http://purl.uniprot.org/core/volume | "69"xsd:string |