| http://purl.uniprot.org/citations/12686464 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12686464 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12686464 | http://www.w3.org/2000/01/rdf-schema#comment | "Relaxin 3 has been reported recently as a member of the insulin/IGF/relaxin family. To clarify the function of relaxin 3, we prepared recombinant human relaxin 3 using a mouse adrenocorticotrophic hormone (ACTH)-secreting cell line, AtT20. To detect a mature form of recombinant human relaxin 3, a competitive enzyme immunoassay (EIA) was developed using a monoclonal antibody (mAb; HK4-144-10), which was raised for the N-terminal peptide of human relaxin 3 A-chain. We detected immunoreactive (ir-) relaxin 3 in the culture supernatant of AtT20 cells stably transfected with human relaxin 3 cDNA. After treatment with 5 microM forskolin for 3 days, the concentration of the ir-relaxin 3 in the culture supernatant reached 12 nM. Ir-relaxin 3 was purified from the culture supernatant by a combination of various chromatographies. By analyses of N-terminal amino acid sequence and electrospray ionization mass spectrometry (ESI-MS), we confirmed that the purified material was a mature form of human relaxin 3. The recombinant human relaxin 3 thereby obtained increased intracellular cAMP production in THP-1 cells. Our results demonstrate that the expression of relaxin 3 cDNA in AtT20 cells is a useful tool to produce a bioactive and mature form of relaxin 3."xsd:string |
| http://purl.uniprot.org/citations/12686464 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0167-0115(02)00304-x"xsd:string |
| http://purl.uniprot.org/citations/12686464 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0167-0115(02)00304-x"xsd:string |
| http://purl.uniprot.org/citations/12686464 | http://purl.uniprot.org/core/author | "Harada M."xsd:string |
| http://purl.uniprot.org/citations/12686464 | http://purl.uniprot.org/core/author | "Harada M."xsd:string |
| http://purl.uniprot.org/citations/12686464 | http://purl.uniprot.org/core/author | "Fujino M."xsd:string |
| http://purl.uniprot.org/citations/12686464 | http://purl.uniprot.org/core/author | "Fujino M."xsd:string |
| http://purl.uniprot.org/citations/12686464 | http://purl.uniprot.org/core/author | "Ito Y."xsd:string |
| http://purl.uniprot.org/citations/12686464 | http://purl.uniprot.org/core/author | "Ito Y."xsd:string |
| http://purl.uniprot.org/citations/12686464 | http://purl.uniprot.org/core/author | "Onda H."xsd:string |
| http://purl.uniprot.org/citations/12686464 | http://purl.uniprot.org/core/author | "Onda H."xsd:string |
| http://purl.uniprot.org/citations/12686464 | http://purl.uniprot.org/core/author | "Suzuki N."xsd:string |
| http://purl.uniprot.org/citations/12686464 | http://purl.uniprot.org/core/author | "Suzuki N."xsd:string |
| http://purl.uniprot.org/citations/12686464 | http://purl.uniprot.org/core/author | "Nishi K."xsd:string |
| http://purl.uniprot.org/citations/12686464 | http://purl.uniprot.org/core/author | "Nishi K."xsd:string |
| http://purl.uniprot.org/citations/12686464 | http://purl.uniprot.org/core/author | "Asano T."xsd:string |
| http://purl.uniprot.org/citations/12686464 | http://purl.uniprot.org/core/author | "Asano T."xsd:string |
| http://purl.uniprot.org/citations/12686464 | http://purl.uniprot.org/core/author | "Ishibashi Y."xsd:string |
| http://purl.uniprot.org/citations/12686464 | http://purl.uniprot.org/core/author | "Ishibashi Y."xsd:string |
| http://purl.uniprot.org/citations/12686464 | http://purl.uniprot.org/core/author | "Fujisawa Y."xsd:string |
| http://purl.uniprot.org/citations/12686464 | http://purl.uniprot.org/core/author | "Fujisawa Y."xsd:string |
| http://purl.uniprot.org/citations/12686464 | http://purl.uniprot.org/core/author | "Hinuma S."xsd:string |
| http://purl.uniprot.org/citations/12686464 | http://purl.uniprot.org/core/author | "Hinuma S."xsd:string |