| http://purl.uniprot.org/citations/12702500 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12702500 | http://www.w3.org/2000/01/rdf-schema#comment | "The CCAAT/enhancer binding protein-alpha (C/EBPalpha) is a transcription factor required for differentiation of myeloid progenitors. In addition to specific DNA binding, C/EBPalpha is also involved in protein-protein interactions, some of which (p21, Cdk2/Cdk4, E2F) appear to be required for inhibition of proliferation and possibly differentiation. To investigate the mechanisms of C/EBPalpha-induced granulocytic differentiation, we generated C/EBPalpha mutants reportedly defective in DNA binding, transactivation, and Cdk2/Cdk4 and E2F interaction and assessed their effects in a myeloid precursor cell line, primary bone marrow and C/EBPalpha knockout fetal liver precursor cells. We show here that the DNA binding-deficient Lys298Glu mutant, the E2F binding-deficient basic region mutant 2 (BRM-2) carrying the Ile294Ala and Arg297Ala substitutions, and the transactivation-deficient N-terminus truncated p30 mutant all fail to promote differentiation on ectopic expression in myeloid precursor cells. By contrast, ectopic expression of the Cdk2/Cdk4 interaction-deficient Delta177-191 mutant promotes differentiation and induces gene expression as effectively as wild-type C/EBPalpha. Thus, the integrity of the transactivation and DNA binding domains, but not of the Cdk2/Cdk4 interaction region, is necessary for C/EBPalpha-induced differentiation. Since the E2F binding-deficient BRM-2 mutant interacted with E2F-1 but failed to activate gene expression, our results lend support to the hypothesis that activation of gene transcription is the determining factor in C/EBPalpha-dependent differentiation."xsd:string |
| http://purl.uniprot.org/citations/12702500 | http://purl.org/dc/terms/identifier | "doi:10.1182/blood-2003-02-0477"xsd:string |
| http://purl.uniprot.org/citations/12702500 | http://purl.uniprot.org/core/author | "Calabretta B."xsd:string |
| http://purl.uniprot.org/citations/12702500 | http://purl.uniprot.org/core/author | "Perrotti D."xsd:string |
| http://purl.uniprot.org/citations/12702500 | http://purl.uniprot.org/core/author | "Keeshan K."xsd:string |
| http://purl.uniprot.org/citations/12702500 | http://purl.uniprot.org/core/author | "Santilli G."xsd:string |
| http://purl.uniprot.org/citations/12702500 | http://purl.uniprot.org/core/author | "Corradini F."xsd:string |
| http://purl.uniprot.org/citations/12702500 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12702500 | http://purl.uniprot.org/core/name | "Blood"xsd:string |
| http://purl.uniprot.org/citations/12702500 | http://purl.uniprot.org/core/pages | "1267-1275"xsd:string |
| http://purl.uniprot.org/citations/12702500 | http://purl.uniprot.org/core/title | "Transcription activation function of C/EBPalpha is required for induction of granulocytic differentiation."xsd:string |
| http://purl.uniprot.org/citations/12702500 | http://purl.uniprot.org/core/volume | "102"xsd:string |
| http://purl.uniprot.org/citations/12702500 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12702500 |
| http://purl.uniprot.org/citations/12702500 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12702500 |
| http://purl.uniprot.org/uniprot/#_A0A0U1RPE0-mappedCitation-12702500 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12702500 |
| http://purl.uniprot.org/uniprot/#_Q14186-mappedCitation-12702500 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12702500 |
| http://purl.uniprot.org/uniprot/#_Q14188-mappedCitation-12702500 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12702500 |
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| http://purl.uniprot.org/uniprot/#_Q01094-mappedCitation-12702500 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12702500 |
| http://purl.uniprot.org/uniprot/Q3U2H8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12702500 |
| http://purl.uniprot.org/uniprot/A0A0U1RPE0 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12702500 |
| http://purl.uniprot.org/uniprot/P53566 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12702500 |