| http://purl.uniprot.org/citations/12702713 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12702713 | http://www.w3.org/2000/01/rdf-schema#comment | "The delta subunit of the gamma-aminobutyric acid (A) receptor (GABA(A)R) is expressed postnatally mostly in the cerebellum, thalamus, and dentate gyrus. Previous studies in mice with a targeted disruption of the delta subunit revealed a considerable attenuation of behavioral responses to neuroactive steroids but not to other neuromodulatory drugs. Here we show that delta subunit loss leads to a concomitant reduction in hippocampal alpha4 subunit levels. These changes were accompanied by faster decay of evoked inhibitory postsynaptic potentials (IPSPs) in dentate granule neurons of -/-mutants (decay tau = 25 ms) compared with +/+ controls (tau = 50 ms). Furthermore, the GABA(A)R-mediated miniature inhibitory postsynaptic currents (mIPSCs) also decayed faster in delta-mutants (tau = 6.3 ms) than controls (tau = 7.2 ms) and had decreased frequency (controls, 10.5 Hz; mutants, 6.6 Hz). Prolongation of mIPSCs by the neuroactive steroid anesthetic, alphaxalone (1-10 microM), was smaller in delta-mutants (at 10 microM, 65% increase) compared with +/+ littermates (308% increase). In competition binding experiments, alphaxalone (0.03-1 microM) modulation of [35S]t-butylbicyclophosphorothionate binding was reduced in delta-mutant brain homogenates, indicating that the decreased alphaxalone effects on mIPSCs were due to changes in the GABA(A)R protein. Faster decay of evoked IPSPs and mIPSCs in delta-mutants suggests presence of the delta subunit at both synaptic and extrasynaptic GABA(A)Rs. Decreased synaptic and extrasynaptic inhibition likely contributes to the pro-epileptic phenotype of delta-mutants. Reduced neurosteroid sensitivity might also contribute to seizure susceptibility. While the simplest explanation is that delta subunit-containing GABA(A)Rs represent the actual target of neurosteroids, it is possible that the behavioral and physiological sensitivity to neuroactive steroids is indirectly altered in the delta -/- mice."xsd:string |
| http://purl.uniprot.org/citations/12702713 | http://purl.org/dc/terms/identifier | "doi:10.1152/jn.01022.2002"xsd:string |
| http://purl.uniprot.org/citations/12702713 | http://purl.uniprot.org/core/author | "Li Z."xsd:string |
| http://purl.uniprot.org/citations/12702713 | http://purl.uniprot.org/core/author | "Liang J."xsd:string |
| http://purl.uniprot.org/citations/12702713 | http://purl.uniprot.org/core/author | "Olsen R.W."xsd:string |
| http://purl.uniprot.org/citations/12702713 | http://purl.uniprot.org/core/author | "Homanics G.E."xsd:string |
| http://purl.uniprot.org/citations/12702713 | http://purl.uniprot.org/core/author | "Mihalek R.M."xsd:string |
| http://purl.uniprot.org/citations/12702713 | http://purl.uniprot.org/core/author | "Spigelman I."xsd:string |
| http://purl.uniprot.org/citations/12702713 | http://purl.uniprot.org/core/author | "Cagetti E."xsd:string |
| http://purl.uniprot.org/citations/12702713 | http://purl.uniprot.org/core/author | "Samzadeh S."xsd:string |
| http://purl.uniprot.org/citations/12702713 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12702713 | http://purl.uniprot.org/core/name | "J Neurophysiol"xsd:string |
| http://purl.uniprot.org/citations/12702713 | http://purl.uniprot.org/core/pages | "903-910"xsd:string |
| http://purl.uniprot.org/citations/12702713 | http://purl.uniprot.org/core/title | "Reduced inhibition and sensitivity to neurosteroids in hippocampus of mice lacking the GABA(A) receptor delta subunit."xsd:string |
| http://purl.uniprot.org/citations/12702713 | http://purl.uniprot.org/core/volume | "90"xsd:string |
| http://purl.uniprot.org/citations/12702713 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12702713 |
| http://purl.uniprot.org/citations/12702713 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12702713 |
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| http://purl.uniprot.org/uniprot/#_P22933-mappedCitation-12702713 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12702713 |
| http://purl.uniprot.org/uniprot/#_Q8VBV6-mappedCitation-12702713 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12702713 |
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| http://purl.uniprot.org/uniprot/Q14AH9 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12702713 |