| http://purl.uniprot.org/citations/12711671 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12711671 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12711671 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/12711671 | http://www.w3.org/2000/01/rdf-schema#comment | "tRNA 3' processing endoribonuclease (3' tRNase) is an enzyme responsible for the removal of a 3' trailer from precursor tRNA (pre-tRNA). We purified approximately 85 kDa 3' tRNase from pig liver and determined its partial sequences. BLAST search of them suggested that the enzyme was the product of a candidate human prostate cancer susceptibility gene, ELAC2, the biological function of which was totally unknown. We cloned a human ELAC2 cDNA and expressed the ELAC2 protein in Escherichia coli. The recombinant ELAC2 was able to cleave human pre-tRNA(Arg) efficiently. The 3' tRNase activity of the yeast ortholog YKR079C was also observed. The C-terminal half of human ELAC2 was able to remove a 3' trailer from pre-tRNA(Arg), while the N-terminal half failed to do so. In the human genome exists a gene, ELAC1, which seems to correspond to the C-terminal half of 3' tRNase from ELAC2. We showed that human ELAC1 also has 3'-tRNase activity. Furthermore, we examined eight ELAC2 variants that seem to be associated with the occurrence of prostate cancer for 3'-tRNase activity. Seven ELAC2 variants which contain one to three amino acid substitutions showed efficient 3'-tRNase activities, while one truncated variant, which lacked a C-terminal half region, had no activity."xsd:string |
| http://purl.uniprot.org/citations/12711671 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkg337"xsd:string |
| http://purl.uniprot.org/citations/12711671 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkg337"xsd:string |
| http://purl.uniprot.org/citations/12711671 | http://purl.uniprot.org/core/author | "Takagi M."xsd:string |
| http://purl.uniprot.org/citations/12711671 | http://purl.uniprot.org/core/author | "Takagi M."xsd:string |
| http://purl.uniprot.org/citations/12711671 | http://purl.uniprot.org/core/author | "Takaku H."xsd:string |
| http://purl.uniprot.org/citations/12711671 | http://purl.uniprot.org/core/author | "Takaku H."xsd:string |
| http://purl.uniprot.org/citations/12711671 | http://purl.uniprot.org/core/author | "Minagawa A."xsd:string |
| http://purl.uniprot.org/citations/12711671 | http://purl.uniprot.org/core/author | "Minagawa A."xsd:string |
| http://purl.uniprot.org/citations/12711671 | http://purl.uniprot.org/core/author | "Nashimoto M."xsd:string |
| http://purl.uniprot.org/citations/12711671 | http://purl.uniprot.org/core/author | "Nashimoto M."xsd:string |
| http://purl.uniprot.org/citations/12711671 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12711671 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12711671 | http://purl.uniprot.org/core/name | "Nucleic Acids Res."xsd:string |
| http://purl.uniprot.org/citations/12711671 | http://purl.uniprot.org/core/name | "Nucleic Acids Res."xsd:string |
| http://purl.uniprot.org/citations/12711671 | http://purl.uniprot.org/core/pages | "2272-2278"xsd:string |
| http://purl.uniprot.org/citations/12711671 | http://purl.uniprot.org/core/pages | "2272-2278"xsd:string |
| http://purl.uniprot.org/citations/12711671 | http://purl.uniprot.org/core/title | "A candidate prostate cancer susceptibility gene encodes tRNA 3' processing endoribonuclease."xsd:string |
| http://purl.uniprot.org/citations/12711671 | http://purl.uniprot.org/core/title | "A candidate prostate cancer susceptibility gene encodes tRNA 3' processing endoribonuclease."xsd:string |
| http://purl.uniprot.org/citations/12711671 | http://purl.uniprot.org/core/volume | "31"xsd:string |
| http://purl.uniprot.org/citations/12711671 | http://purl.uniprot.org/core/volume | "31"xsd:string |
| http://purl.uniprot.org/citations/12711671 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12711671 |