| http://purl.uniprot.org/citations/12724402 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12724402 | http://www.w3.org/2000/01/rdf-schema#comment | "Friend leukemia integration 1 (Fli-1) is a member of the Ets family of transcriptional activators that has been shown to be an important regulator during megakaryocytic differentiation. We undertook a two-hybrid screen of a K562 cDNA library to identify transcription factors that interacted with Fli-1 and were potential regulators of megakaryocyte development. Here we report the physical interaction of Fli-1 with GATA-1, a well-characterized, zinc finger transcription factor critical for both erythroid and megakaryocytic differentiation. We map the minimal domains required for the interaction and show that the zinc fingers of GATA-1 interact with the Ets domain of Fli-1. GATA-1 has previously been shown to interact with the Ets domain of the Fli-1-related protein PU.1, and the two proteins appear to inhibit each other's activity. In contrast, we demonstrate that GATA-1 and Fli-1 synergistically activate the megakaryocyte-specific promoters GPIX and GPIbalpha in transient transfections. Quantitative electrophoretic mobility shift assays using oligonucleotides derived from the GPIX promoter containing Ets and GATA binding motifs reveal that Fli-1 and GATA-1 exhibit cooperative DNA binding in which the binding of GATA-1 to DNA is increased approximately 26-fold in the presence of Fli-1 (from 4.2 to 0.16 nM), providing a mechanism for the observed transcriptional synergy. To test the effect on endogenous genes, we stably overexpressed Fli-1 in K562 cells, a line rich in GATA-1. Overexpression of Fli-1 induced the expression of the endogenous GPIX and GPIbalpha genes as measured by Northern blot and fluorescence-activated cell sorter analysis. This work suggests that Fli-1 and GATA-1 work together to activate the expression of genes associated with the terminal differentiation of megakaryocytes."xsd:string |
| http://purl.uniprot.org/citations/12724402 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.23.10.3427-3441.2003"xsd:string |
| http://purl.uniprot.org/citations/12724402 | http://purl.uniprot.org/core/author | "Holmes M.L."xsd:string |
| http://purl.uniprot.org/citations/12724402 | http://purl.uniprot.org/core/author | "Newton A."xsd:string |
| http://purl.uniprot.org/citations/12724402 | http://purl.uniprot.org/core/author | "Crossley M."xsd:string |
| http://purl.uniprot.org/citations/12724402 | http://purl.uniprot.org/core/author | "Hogg P.J."xsd:string |
| http://purl.uniprot.org/citations/12724402 | http://purl.uniprot.org/core/author | "Chong B.H."xsd:string |
| http://purl.uniprot.org/citations/12724402 | http://purl.uniprot.org/core/author | "Khachigian L.M."xsd:string |
| http://purl.uniprot.org/citations/12724402 | http://purl.uniprot.org/core/author | "Eisbacher M."xsd:string |
| http://purl.uniprot.org/citations/12724402 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12724402 | http://purl.uniprot.org/core/name | "Mol Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/12724402 | http://purl.uniprot.org/core/pages | "3427-3441"xsd:string |
| http://purl.uniprot.org/citations/12724402 | http://purl.uniprot.org/core/title | "Protein-protein interaction between Fli-1 and GATA-1 mediates synergistic expression of megakaryocyte-specific genes through cooperative DNA binding."xsd:string |
| http://purl.uniprot.org/citations/12724402 | http://purl.uniprot.org/core/volume | "23"xsd:string |
| http://purl.uniprot.org/citations/12724402 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12724402 |
| http://purl.uniprot.org/citations/12724402 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12724402 |
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| http://purl.uniprot.org/uniprot/#_R9UV10-mappedCitation-12724402 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12724402 |
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| http://purl.uniprot.org/uniprot/#_Q9UEE3-mappedCitation-12724402 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12724402 |
| http://purl.uniprot.org/uniprot/Q9UEE2 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12724402 |
| http://purl.uniprot.org/uniprot/Q9UEE3 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12724402 |