| http://purl.uniprot.org/citations/12724526 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12724526 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12724526 | http://www.w3.org/2000/01/rdf-schema#comment | "Mycobacterium tuberculosis, the causative agent of human tuberculosis, is unique among bacterial pathogens in that it displays a wide array of complex lipids and lipoglycans on its cell surface. One of the more remarkable lipids is a sulfated glycolipid, termed sulfolipid-1 (SL-1), which is thought to mediate specific host-pathogen interactions during infection. However, a direct role for SL-1 in M. tuberculosis virulence has not been established. Here we show that MmpL8, a member of a large family of predicted lipid transporters in M. tuberculosis, is required for SL-1 production. The accumulation of an SL-1 precursor, termed SL(1278), in mmpL8 mutant cells indicates that MmpL8 is necessary for an intermediate step in the SL-1 biosynthesis pathway. We use a novel fractionation procedure to demonstrate that SL-1 is present on the cell surface, whereas SL(1278) is found exclusively in more internal layers. Importantly, we show that mmpL8 mutants are attenuated for growth in a mouse model of tuberculosis. However, SL-1 per se is not required for establishing infection as pks2 mutants, which are defective in an earlier step in SL-1 biosynthesis, have no obvious growth defect. Thus, we hypothesize that either MmpL8 transports molecules in addition to SL-1 that mediate host-pathogen interactions or the accumulation of SL(1278) in mmpL8 mutant cells interferes with other pathways required for growth during the early stages of infection."xsd:string |
| http://purl.uniprot.org/citations/12724526 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1030024100"xsd:string |
| http://purl.uniprot.org/citations/12724526 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1030024100"xsd:string |
| http://purl.uniprot.org/citations/12724526 | http://purl.uniprot.org/core/author | "Cox J.S."xsd:string |
| http://purl.uniprot.org/citations/12724526 | http://purl.uniprot.org/core/author | "Cox J.S."xsd:string |
| http://purl.uniprot.org/citations/12724526 | http://purl.uniprot.org/core/author | "Mougous J.D."xsd:string |
| http://purl.uniprot.org/citations/12724526 | http://purl.uniprot.org/core/author | "Mougous J.D."xsd:string |
| http://purl.uniprot.org/citations/12724526 | http://purl.uniprot.org/core/author | "Leary J.A."xsd:string |
| http://purl.uniprot.org/citations/12724526 | http://purl.uniprot.org/core/author | "Leary J.A."xsd:string |
| http://purl.uniprot.org/citations/12724526 | http://purl.uniprot.org/core/author | "Leavell M.D."xsd:string |
| http://purl.uniprot.org/citations/12724526 | http://purl.uniprot.org/core/author | "Leavell M.D."xsd:string |
| http://purl.uniprot.org/citations/12724526 | http://purl.uniprot.org/core/author | "Bertozzi C.R."xsd:string |
| http://purl.uniprot.org/citations/12724526 | http://purl.uniprot.org/core/author | "Bertozzi C.R."xsd:string |
| http://purl.uniprot.org/citations/12724526 | http://purl.uniprot.org/core/author | "Converse S.E."xsd:string |
| http://purl.uniprot.org/citations/12724526 | http://purl.uniprot.org/core/author | "Converse S.E."xsd:string |
| http://purl.uniprot.org/citations/12724526 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12724526 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12724526 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/12724526 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/12724526 | http://purl.uniprot.org/core/pages | "6121-6126"xsd:string |
| http://purl.uniprot.org/citations/12724526 | http://purl.uniprot.org/core/pages | "6121-6126"xsd:string |
| http://purl.uniprot.org/citations/12724526 | http://purl.uniprot.org/core/title | "MmpL8 is required for sulfolipid-1 biosynthesis and Mycobacterium tuberculosis virulence."xsd:string |
| http://purl.uniprot.org/citations/12724526 | http://purl.uniprot.org/core/title | "MmpL8 is required for sulfolipid-1 biosynthesis and Mycobacterium tuberculosis virulence."xsd:string |