| http://purl.uniprot.org/citations/12727301 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12727301 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12727301 | http://www.w3.org/2000/01/rdf-schema#comment | "Venom from the parasitoid wasp Pimpla hypochondriaca contains numerous proteins, has potent in vitro anti-haemocytic properties, and disrupts host encapsulation responses. By sequencing 500 cDNAs randomly isolated from a venom gland library, we have identified 60 clones that encode proteins containing potential secretory signal sequences. To identify cDNAs encoding particular venom proteins, N-terminal amino acid sequences were determined for large (>30 kDa) venom proteins that had been separated using a combination of gel filtration and SDS-PAGE. We describe five of these cDNAs, which encoded residues that matched with the N-terminal sequences of previously undescribed venom proteins. cDNAs vpr1 and vpr3 encoded related proteins of approximately 32 kDa that were found in widely different fractions of gel filtration-separated venom. Neither vpr1 nor vpr3 were closely related to any other protein in the GenBank database, suggesting that they are highly specialised venom components. vpr2 encoded a 57-kDa polypeptide that was similar to a Drosophila protein, of unknown function, which lacks a signal sequence. A fourth clone, tre1, encoded a 61-kDa protein with extensive sequence similarity to trehalases. The 76-kDa sequence encoded by lac1 contained three regions which were very similar to histidine-rich copper-binding motifs, and could be aligned with the laccase from the fungus Coprinus cinereus. This study represents a significant step towards a holistic view of the molecular composition of a parasitoid wasp venom."xsd:string |
| http://purl.uniprot.org/citations/12727301 | http://purl.org/dc/terms/identifier | "doi:10.1016/s1532-0456(03)00041-3"xsd:string |
| http://purl.uniprot.org/citations/12727301 | http://purl.org/dc/terms/identifier | "doi:10.1016/s1532-0456(03)00041-3"xsd:string |
| http://purl.uniprot.org/citations/12727301 | http://purl.uniprot.org/core/author | "Keen J.N."xsd:string |
| http://purl.uniprot.org/citations/12727301 | http://purl.uniprot.org/core/author | "Keen J.N."xsd:string |
| http://purl.uniprot.org/citations/12727301 | http://purl.uniprot.org/core/author | "Smith I."xsd:string |
| http://purl.uniprot.org/citations/12727301 | http://purl.uniprot.org/core/author | "Smith I."xsd:string |
| http://purl.uniprot.org/citations/12727301 | http://purl.uniprot.org/core/author | "Weaver R.J."xsd:string |
| http://purl.uniprot.org/citations/12727301 | http://purl.uniprot.org/core/author | "Weaver R.J."xsd:string |
| http://purl.uniprot.org/citations/12727301 | http://purl.uniprot.org/core/author | "Conyers C.M."xsd:string |
| http://purl.uniprot.org/citations/12727301 | http://purl.uniprot.org/core/author | "Conyers C.M."xsd:string |
| http://purl.uniprot.org/citations/12727301 | http://purl.uniprot.org/core/author | "MacNicoll A.D."xsd:string |
| http://purl.uniprot.org/citations/12727301 | http://purl.uniprot.org/core/author | "MacNicoll A.D."xsd:string |
| http://purl.uniprot.org/citations/12727301 | http://purl.uniprot.org/core/author | "Parkinson N.M."xsd:string |
| http://purl.uniprot.org/citations/12727301 | http://purl.uniprot.org/core/author | "Parkinson N.M."xsd:string |
| http://purl.uniprot.org/citations/12727301 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12727301 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12727301 | http://purl.uniprot.org/core/name | "Comp. Biochem. Physiol."xsd:string |
| http://purl.uniprot.org/citations/12727301 | http://purl.uniprot.org/core/name | "Comp. Biochem. Physiol."xsd:string |
| http://purl.uniprot.org/citations/12727301 | http://purl.uniprot.org/core/pages | "513-520"xsd:string |
| http://purl.uniprot.org/citations/12727301 | http://purl.uniprot.org/core/pages | "513-520"xsd:string |
| http://purl.uniprot.org/citations/12727301 | http://purl.uniprot.org/core/title | "cDNAs encoding large venom proteins from the parasitoid wasp Pimpla hypochondriaca identified by random sequence analysis."xsd:string |
| http://purl.uniprot.org/citations/12727301 | http://purl.uniprot.org/core/title | "cDNAs encoding large venom proteins from the parasitoid wasp Pimpla hypochondriaca identified by random sequence analysis."xsd:string |