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http://purl.uniprot.org/citations/12730876http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/12730876http://www.w3.org/2000/01/rdf-schema#comment"

Background & aims

The mechanisms of interleukin-11 (IL-11) cytoprotection in intestinal epithelial injury are largely unknown. IL-11 protects barrier integrity during oxidant stress, a common endpoint of numerous types of intestinal injury including ischemia and immune-mediated inflammation. Because heat shock proteins (hsp) are cytoprotective in intestinal epithelia, we hypothesized that IL-11-conferred cytoprotection is mediated by inducible hsps.

Methods

IL-11 receptor (IL-11R) activation was determined using phospho-specific antibodies to STAT3. IL-11 induction of hsp72 and hsp25 was determined by immunoblot in IEC-18 crypt and young adult mouse colon colonic epithelial cells. Epithelial resistance to oxidant injury by monochloramine was determined by (51)Cr release. Stable hsp anti-sense IEC-18 cell clones were obtained by electroporation and hygromycin B selection. The IL-11 effect on hsp25 distribution was characterized by analysis of Triton x-100 insoluble fractions, 2-D isoelectric focusing gels, and confocal microscopy.

Results

IL-11R signaling was detected in all cells under study. IL-11 induces hsp25 in an intestinal epithelial-specific manner that significantly preserves cellular viability in the presence of monochloramine. This effect was significantly reversed in intestinal epithelia stably expressing anti-sense to hsp25. IL-11 induced a shift of hsp25 to Triton x-100 insoluble fractions containing cytoskeletal elements, which was not associated with altered hsp25 phosphorylation. The shift was not paralleled by increased hsp25 co-localization with F-actin by confocal microscopy.

Conclusions

The induction of hsp25 by IL-11 confers epithelial-specific cytoprotection that is independent of phosphorylation-dependent co-localization of hsp25 to F-actin, thereby contributing to the protective effects of IL-11 in models of intestinal epithelial injury."xsd:string
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http://purl.uniprot.org/citations/12730876http://purl.uniprot.org/core/author"Tang J."xsd:string
http://purl.uniprot.org/citations/12730876http://purl.uniprot.org/core/author"Musch M.W."xsd:string
http://purl.uniprot.org/citations/12730876http://purl.uniprot.org/core/author"Chang E.B."xsd:string
http://purl.uniprot.org/citations/12730876http://purl.uniprot.org/core/author"Walsh-Reitz M.M."xsd:string
http://purl.uniprot.org/citations/12730876http://purl.uniprot.org/core/author"Ropeleski M.J."xsd:string
http://purl.uniprot.org/citations/12730876http://purl.uniprot.org/core/date"2003"xsd:gYear
http://purl.uniprot.org/citations/12730876http://purl.uniprot.org/core/name"Gastroenterology"xsd:string
http://purl.uniprot.org/citations/12730876http://purl.uniprot.org/core/pages"1358-1368"xsd:string
http://purl.uniprot.org/citations/12730876http://purl.uniprot.org/core/title"Interleukin-11-induced heat shock protein 25 confers intestinal epithelial-specific cytoprotection from oxidant stress."xsd:string
http://purl.uniprot.org/citations/12730876http://purl.uniprot.org/core/volume"124"xsd:string
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