| http://purl.uniprot.org/citations/12734178 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12734178 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12734178 | http://www.w3.org/2000/01/rdf-schema#comment | "Type III protein secretion (TTS) is catalyzed by translocases that span both membranes of Gram-negative bacteria. A hydrophilic TTS component homologous to F1/V1-ATPases is ubiquitous and essential for secretion. We show that hrcN encodes the putative TTS ATPase of Pseudomonas syringae pathovar phaseolicola and that HrcN is a peripheral protein that assembles in clusters at the membrane. A decahistidinyl HrcN derivative was overexpressed in Escherichia coli and purified to homogeneity in a folded state. Hydrodynamic analysis, cross-linking, and electron microscopy revealed four distinct HrcN forms: I, 48 kDa (monomer); II, approximately 300 kDa (putative hexamer); III, 575 kDa (dodecamer); and IV, approximately 3.5 MDa. Form III is the predominant form of HrcN at the membrane, and its ATPase activity is dramatically stimulated (>700-fold) over the basal activity of Form I. We propose that TTS ATPases catalyze protein translocation as activated homo-oligomers at the plasma membrane."xsd:string |
| http://purl.uniprot.org/citations/12734178 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m301903200"xsd:string |
| http://purl.uniprot.org/citations/12734178 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m301903200"xsd:string |
| http://purl.uniprot.org/citations/12734178 | http://purl.uniprot.org/core/author | "Engel A."xsd:string |
| http://purl.uniprot.org/citations/12734178 | http://purl.uniprot.org/core/author | "Engel A."xsd:string |
| http://purl.uniprot.org/citations/12734178 | http://purl.uniprot.org/core/author | "Economou A."xsd:string |
| http://purl.uniprot.org/citations/12734178 | http://purl.uniprot.org/core/author | "Economou A."xsd:string |
| http://purl.uniprot.org/citations/12734178 | http://purl.uniprot.org/core/author | "Stahlberg H."xsd:string |
| http://purl.uniprot.org/citations/12734178 | http://purl.uniprot.org/core/author | "Stahlberg H."xsd:string |
| http://purl.uniprot.org/citations/12734178 | http://purl.uniprot.org/core/author | "Pugsley A.P."xsd:string |
| http://purl.uniprot.org/citations/12734178 | http://purl.uniprot.org/core/author | "Pugsley A.P."xsd:string |
| http://purl.uniprot.org/citations/12734178 | http://purl.uniprot.org/core/author | "Lustig A."xsd:string |
| http://purl.uniprot.org/citations/12734178 | http://purl.uniprot.org/core/author | "Lustig A."xsd:string |
| http://purl.uniprot.org/citations/12734178 | http://purl.uniprot.org/core/author | "Mansfield J."xsd:string |
| http://purl.uniprot.org/citations/12734178 | http://purl.uniprot.org/core/author | "Mansfield J."xsd:string |
| http://purl.uniprot.org/citations/12734178 | http://purl.uniprot.org/core/author | "Panopoulos N.J."xsd:string |
| http://purl.uniprot.org/citations/12734178 | http://purl.uniprot.org/core/author | "Panopoulos N.J."xsd:string |
| http://purl.uniprot.org/citations/12734178 | http://purl.uniprot.org/core/author | "Tampakaki A.P."xsd:string |
| http://purl.uniprot.org/citations/12734178 | http://purl.uniprot.org/core/author | "Tampakaki A.P."xsd:string |
| http://purl.uniprot.org/citations/12734178 | http://purl.uniprot.org/core/author | "Pozidis C."xsd:string |
| http://purl.uniprot.org/citations/12734178 | http://purl.uniprot.org/core/author | "Pozidis C."xsd:string |
| http://purl.uniprot.org/citations/12734178 | http://purl.uniprot.org/core/author | "Politou A.S."xsd:string |
| http://purl.uniprot.org/citations/12734178 | http://purl.uniprot.org/core/author | "Politou A.S."xsd:string |