| http://purl.uniprot.org/citations/12734182 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12734182 | http://www.w3.org/2000/01/rdf-schema#comment | "Insulin plays a central role in the regulation of glucose homeostasis in part by stimulating glucose uptake and glycogen synthesis. The serine/threonine protein kinase Akt has been proposed to mediate insulin signaling in several processes. However, it is unclear whether Akt is involved in insulin-stimulated glucose uptake and which isoforms of Akt are responsible for each insulin action. We confirmed that expression of a constitutively active Akt, using an adenoviral expression vector, promoted translocation of glucose transporter 4 (GLUT4) to plasma membrane, 2-deoxyglucose (2-DG) uptake, and glycogen synthesis in both Chinese hamster ovary cells and 3T3-L1 adipocytes. Inhibition of Akt either by adenoviral expression of a dominant negative Akt or by the introduction of synthetic 21-mer short interference RNA against Akt markedly reduced insulin-stimulated GLUT4 translocation, 2-DG uptake, and glycogen synthesis. Experiments with isoform-specific short interference RNA revealed that Akt2, and Akt1 to a lesser extent, has an essential role in insulin-stimulated GLUT4 translocation and 2-DG uptake in both cell lines, whereas Akt1 and Akt2 contribute equally to insulin-stimulated glycogen synthesis. These data suggest a prerequisite role of Akt in insulin-stimulated glucose uptake and distinct functions among Akt isoforms."xsd:string |
| http://purl.uniprot.org/citations/12734182 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m302094200"xsd:string |
| http://purl.uniprot.org/citations/12734182 | http://purl.uniprot.org/core/author | "Cantley L.C."xsd:string |
| http://purl.uniprot.org/citations/12734182 | http://purl.uniprot.org/core/author | "Gotoh Y."xsd:string |
| http://purl.uniprot.org/citations/12734182 | http://purl.uniprot.org/core/author | "Matsushima R."xsd:string |
| http://purl.uniprot.org/citations/12734182 | http://purl.uniprot.org/core/author | "Masuyama N."xsd:string |
| http://purl.uniprot.org/citations/12734182 | http://purl.uniprot.org/core/author | "Obata T."xsd:string |
| http://purl.uniprot.org/citations/12734182 | http://purl.uniprot.org/core/author | "Ebina Y."xsd:string |
| http://purl.uniprot.org/citations/12734182 | http://purl.uniprot.org/core/author | "Shiota H."xsd:string |
| http://purl.uniprot.org/citations/12734182 | http://purl.uniprot.org/core/author | "Kishi K."xsd:string |
| http://purl.uniprot.org/citations/12734182 | http://purl.uniprot.org/core/author | "Katome T."xsd:string |
| http://purl.uniprot.org/citations/12734182 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12734182 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/12734182 | http://purl.uniprot.org/core/pages | "28312-28323"xsd:string |
| http://purl.uniprot.org/citations/12734182 | http://purl.uniprot.org/core/title | "Use of RNA interference-mediated gene silencing and adenoviral overexpression to elucidate the roles of AKT/protein kinase B isoforms in insulin actions."xsd:string |
| http://purl.uniprot.org/citations/12734182 | http://purl.uniprot.org/core/volume | "278"xsd:string |
| http://purl.uniprot.org/citations/12734182 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12734182 |
| http://purl.uniprot.org/citations/12734182 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12734182 |
| http://purl.uniprot.org/uniprot/#_O60343-mappedCitation-12734182 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12734182 |
| http://purl.uniprot.org/uniprot/#_P31749-mappedCitation-12734182 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12734182 |
| http://purl.uniprot.org/uniprot/#_P31751-mappedCitation-12734182 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12734182 |
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| http://purl.uniprot.org/uniprot/Q9UIQ6 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12734182 |
| http://purl.uniprot.org/uniprot/P31749 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12734182 |