| http://purl.uniprot.org/citations/12734358 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12734358 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12734358 | http://www.w3.org/2000/01/rdf-schema#comment | "Studies from our laboratory have revealed a novel mu opiate receptor, mu 3, which is expressed in both vascular tissues and leukocytes. The mu 3 receptor is selective for opiate alkaloids and is insensitive to opioid peptides. We now identify the mu 3 receptor at the molecular level using a 441-bp conserved region of the mu 1 receptor. Sequence analysis of the isolated cDNA suggests that it is a novel, alternatively spliced variant of the mu opiate receptor gene. To determine whether protein expressed from this cDNA exhibits the biochemical characteristics expected of the mu 3 receptor, the cDNA clone was expressed in a heterologous system. At the functional level, COS-1 cells transfected with the mu 3 receptor cDNA exhibited dose-dependent release of NO following treatment with morphine, but not opioid peptides (i.e., Met-enkephalin). Naloxone was able to block the effect of morphine on COS-1 transfected cells. Nontransfected COS-1 cells did not produce NO in the presence of morphine or the opioid peptides at similar concentrations. Receptor binding analysis with [(3)H]dihydromorphine further supports the opiate alkaloid selectivity and opioid peptide insensitivity of this receptor. These data suggest that this new mu opiate receptor cDNA encodes the mu 3 opiate receptor, since it exhibits biochemical characteristics known to be unique to this receptor (opiate alkaloid selective and opioid peptide insensitive). Furthermore, using Northern blot, RT-PCR, and sequence analysis, we have demonstrated the expression of this new mu variant in human vascular tissue, mononuclear cells, polymorphonuclear cells, and human neuroblastoma cells."xsd:string |
| http://purl.uniprot.org/citations/12734358 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.170.10.5118"xsd:string |
| http://purl.uniprot.org/citations/12734358 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.170.10.5118"xsd:string |
| http://purl.uniprot.org/citations/12734358 | http://purl.uniprot.org/core/author | "Stefano G.B."xsd:string |
| http://purl.uniprot.org/citations/12734358 | http://purl.uniprot.org/core/author | "Stefano G.B."xsd:string |
| http://purl.uniprot.org/citations/12734358 | http://purl.uniprot.org/core/author | "Cadet P."xsd:string |
| http://purl.uniprot.org/citations/12734358 | http://purl.uniprot.org/core/author | "Cadet P."xsd:string |
| http://purl.uniprot.org/citations/12734358 | http://purl.uniprot.org/core/author | "Mantione K.J."xsd:string |
| http://purl.uniprot.org/citations/12734358 | http://purl.uniprot.org/core/author | "Mantione K.J."xsd:string |
| http://purl.uniprot.org/citations/12734358 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12734358 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12734358 | http://purl.uniprot.org/core/name | "J. Immunol."xsd:string |
| http://purl.uniprot.org/citations/12734358 | http://purl.uniprot.org/core/name | "J. Immunol."xsd:string |
| http://purl.uniprot.org/citations/12734358 | http://purl.uniprot.org/core/pages | "5118-5123"xsd:string |
| http://purl.uniprot.org/citations/12734358 | http://purl.uniprot.org/core/pages | "5118-5123"xsd:string |
| http://purl.uniprot.org/citations/12734358 | http://purl.uniprot.org/core/title | "Molecular identification and functional expression of mu 3, a novel alternatively spliced variant of the human mu opiate receptor gene."xsd:string |
| http://purl.uniprot.org/citations/12734358 | http://purl.uniprot.org/core/title | "Molecular identification and functional expression of mu 3, a novel alternatively spliced variant of the human mu opiate receptor gene."xsd:string |
| http://purl.uniprot.org/citations/12734358 | http://purl.uniprot.org/core/volume | "170"xsd:string |
| http://purl.uniprot.org/citations/12734358 | http://purl.uniprot.org/core/volume | "170"xsd:string |
| http://purl.uniprot.org/citations/12734358 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12734358 |
| http://purl.uniprot.org/citations/12734358 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12734358 |
| http://purl.uniprot.org/citations/12734358 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12734358 |
| http://purl.uniprot.org/citations/12734358 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12734358 |