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http://purl.uniprot.org/citations/12743567http://www.w3.org/2000/01/rdf-schema#comment"

Background

Intercellular adhesion molecule 3 (ICAM-3) has recently been identified on the surface of eosinophils.

Objective

The purpose of this study was to characterize ICAM-3 expression on eosinophils in response to cytokines and to determine whether ligand binding of ICAM-3 modulates inflammatory responses of eosinophils, as it does in other leukocytes.

Methods

To determine effects of ICAM-3 on eosinophil function, we isolated human eosinophils and used a monoclonal antibody directed against the epitope of ICAM-3 that binds to leukocyte-function antigen-1 to mimic binding of ICAM-3 and this natural ligand. We measured granulocyte-macrophage colony stimulating factor (GM-CSF) production by unstimulated eosinophils and eosinophils stimulated with ionomycin (1 micromol/L), both in the presence and absence of this anti-ICAM-3 antibody.

Results

We found that 99% of eosinophils expressed ICAM-3, regardless of whether allergic symptoms were present or absent. Expression of ICAM-3 was not enhanced by proinflammatory cytokines. Expression of ICAM-3 was reduced in apoptotic cells and in cells incubated with the combination of GM-CSF and tumor necrosis factor-alpha (n = 3). Antibody binding of ICAM-3, which mimics leukocyte-function antigen-1 binding, had no effect on baseline GM-CSF production but reduced by 80% the production of GM-CSF stimulated by ionomycin (control 1969 pg/mL +/-1259 SD versus anti-ICAM-3 396 pg/mL +/-207 SD, n = 8) and reduced GM-CSF mRNA content.

Conclusions

ICAM-3 is highly expressed on the surface of human eosinophils, and downregulation of GM-CSF production by anti-ICAM-3 mAb suggests that ICAM-3 ligation may inhibit eosinophil inflammatory responses and survival."xsd:string
http://purl.uniprot.org/citations/12743567http://purl.org/dc/terms/identifier"doi:10.1067/mai.2003.1393"xsd:string
http://purl.uniprot.org/citations/12743567http://purl.uniprot.org/core/author"Busse W.W."xsd:string
http://purl.uniprot.org/citations/12743567http://purl.uniprot.org/core/author"Gern J.E."xsd:string
http://purl.uniprot.org/citations/12743567http://purl.uniprot.org/core/author"Sedgwick J.B."xsd:string
http://purl.uniprot.org/citations/12743567http://purl.uniprot.org/core/author"Vrtis R.F."xsd:string
http://purl.uniprot.org/citations/12743567http://purl.uniprot.org/core/author"Kessel J.M."xsd:string
http://purl.uniprot.org/citations/12743567http://purl.uniprot.org/core/date"2003"xsd:gYear
http://purl.uniprot.org/citations/12743567http://purl.uniprot.org/core/name"J Allergy Clin Immunol"xsd:string
http://purl.uniprot.org/citations/12743567http://purl.uniprot.org/core/pages"1024-1031"xsd:string
http://purl.uniprot.org/citations/12743567http://purl.uniprot.org/core/title"Ligation of intercellular adhesion molecule 3 inhibits GM-CSF production by human eosinophils."xsd:string
http://purl.uniprot.org/citations/12743567http://purl.uniprot.org/core/volume"111"xsd:string
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