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http://purl.uniprot.org/citations/12753154http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/12753154http://www.w3.org/2000/01/rdf-schema#comment"

Background

The molecular processes leading to mucosal atrophy, regrowth, and functional changes with starvation and refeeding are largely unknown. There are many transcriptional factors that might be related to mucosal atrophy and proliferation. In contrast, we previously reported that H+/peptide transporter and aminopeptidase N messenger RNA in the intestinal mucosa were upregulated during starvation. Therefore, we selected and studied three transcriptional factors: activator protein (AP)-1, Sp1, and hepatocyte nuclear factor (HNF)-1, which not only play important roles for enterocytes proliferation, but also exist in promoter lesions of the brush border enzymes and peptide transporter.

Methods

In the present study, we performed electrophoretic mobility shift assays employing AP-1, Sp1, and HNF-1, and evaluated the changes in the DNA binding activities in rat jejunum during starvation and refeeding.

Results

Two days after starvation, the Sp1 binding activity was significantly decreased to 61.8% as compared with the control level, whereas AP-1 was 121.4% and HNF-1 was 77.5%. Two hours after refeeding, the AP-1 activity was significantly increased to 175.0% as compared with the control level, and the HNF-1 activity was significantly increased to 180.2%. In contrast, the decreased SP1 level did not recover until 24 h after refeeding.

Conclusions

The DNA binding activities of these three transcriptional factors were significantly changed in the rat jejunum during starvation and refeeding. Our results provide insight into the molecular mechanisms of the transcriptional regulations associated with mucosal atrophy, regrowth, and functional changes of the jejunal epithelium in response to starvation and refeeding."xsd:string
http://purl.uniprot.org/citations/12753154http://purl.org/dc/terms/identifier"doi:10.1046/j.1440-1746.2003.02924.x"xsd:string
http://purl.uniprot.org/citations/12753154http://purl.uniprot.org/core/author"Ihara T."xsd:string
http://purl.uniprot.org/citations/12753154http://purl.uniprot.org/core/author"Bamba T."xsd:string
http://purl.uniprot.org/citations/12753154http://purl.uniprot.org/core/author"Fujiyama Y."xsd:string
http://purl.uniprot.org/citations/12753154http://purl.uniprot.org/core/author"Tsujikawa T."xsd:string
http://purl.uniprot.org/citations/12753154http://purl.uniprot.org/core/date"2003"xsd:gYear
http://purl.uniprot.org/citations/12753154http://purl.uniprot.org/core/name"J Gastroenterol Hepatol"xsd:string
http://purl.uniprot.org/citations/12753154http://purl.uniprot.org/core/pages"705-711"xsd:string
http://purl.uniprot.org/citations/12753154http://purl.uniprot.org/core/title"Alterations in the DNA binding activity of transcriptional factors activator protein-1, Sp1, and hepatocyte nuclear factor-1 in rat jejunum during starvation and refeeding."xsd:string
http://purl.uniprot.org/citations/12753154http://purl.uniprot.org/core/volume"18"xsd:string
http://purl.uniprot.org/citations/12753154http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/12753154
http://purl.uniprot.org/citations/12753154http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/12753154
http://purl.uniprot.org/uniprot/P15257#attribution-89AFB5F3DC167E6C938E8BA09221D85Bhttp://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/12753154
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http://purl.uniprot.org/uniprot/#_P15257-mappedCitation-12753154http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/12753154
http://purl.uniprot.org/uniprot/#_Q01714-mappedCitation-12753154http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/12753154
http://purl.uniprot.org/uniprot/#_P17325-mappedCitation-12753154http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/12753154
http://purl.uniprot.org/uniprot/Q01714http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/12753154
http://purl.uniprot.org/uniprot/P17325http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/12753154
http://purl.uniprot.org/uniprot/P15257http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/12753154