| http://purl.uniprot.org/citations/12756531 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12756531 | http://www.w3.org/2000/01/rdf-schema#comment | "Zymocin, a toxic protein complex produced by Kluyveromyces lactis, inhibits cell cycle progression in Saccharomyces cerevisiae. In studying its action, a resistant mutant ( kti14-1) was found to express the tot-phenotype typical of totDelta cells, toxin target (TOT) mutants that are impaired in RNA polymerase II Elongator function. Phenotypic analysis of a kti14-1 tot3Delta double mutant revealed a functional link between KTI14 and TOT/Elongator. Unlike totDelta cells, the kti14-1 mutant is sensitive to the drug methylmethane sulfonate (MMS), indicating that, besides being affected in TOT function, kti14-1 cells are also compromised in DNA repair. Single-copy complementation identified HRR25, which codes for casein kinase I (CKI), as KTI14. Kinase-minus hrr25 mutations (K38A and T176I) conferred zymocin resistance, while deletion of the other yeast CKI genes ( YCK1-3) had no effect. A mutation in KTI14 that truncates the P/Q-rich C-terminus of Hrr25p also dissociates MMS sensitivity from zymocin resistance; this mutant is resistant to the toxin, but shows normal sensitivity to MMS. Thus, although kinase-minus mutations are sufficient to protect yeast cells from zymocin, toxicity is also dependent on the integrity of the C-terminal region of Hrr25p, which has been implicated in determining the substrate specificity or localization of Hrr25p."xsd:string |
| http://purl.uniprot.org/citations/12756531 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00438-003-0807-5"xsd:string |
| http://purl.uniprot.org/citations/12756531 | http://purl.uniprot.org/core/author | "Mehlgarten C."xsd:string |
| http://purl.uniprot.org/citations/12756531 | http://purl.uniprot.org/core/author | "Schaffrath R."xsd:string |
| http://purl.uniprot.org/citations/12756531 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12756531 | http://purl.uniprot.org/core/name | "Mol Genet Genomics"xsd:string |
| http://purl.uniprot.org/citations/12756531 | http://purl.uniprot.org/core/pages | "188-196"xsd:string |
| http://purl.uniprot.org/citations/12756531 | http://purl.uniprot.org/core/title | "Mutant casein kinase I (Hrr25p/Kti14p) abrogates the G1 cell cycle arrest induced by Kluyveromyces lactiszymocin in budding yeast."xsd:string |
| http://purl.uniprot.org/citations/12756531 | http://purl.uniprot.org/core/volume | "269"xsd:string |
| http://purl.uniprot.org/citations/12756531 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12756531 |
| http://purl.uniprot.org/citations/12756531 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12756531 |
| http://purl.uniprot.org/uniprot/#_P29295-mappedCitation-12756531 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12756531 |
| http://purl.uniprot.org/uniprot/P29295 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12756531 |