| http://purl.uniprot.org/citations/12759356 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12759356 | http://www.w3.org/2000/01/rdf-schema#comment | "Bone morphogenetic protein-2 (BMP-2) regulates growth plate chondrogenesis during development and postnatal bone growth, but the control mechanisms of BMP-2 expression in growth plate chondrocytes are unknown. Here we have used both in vitro and in vivo approaches to demonstrate that transcription factor, NF-kappaB, regulates BMP-2 gene expression in chondrocytes. Two putative NF-kappaB response elements were found in the -2712/+165 region of the BMP-2 gene. Cotransfection of mutant I-kappaBalpha expression plasmids with BMP-2 promoter-luciferase reporters into TMC-23 chondrocyte cell line suppressed BMP-2 transcription. Mutations in NF-kappaB response elements in the BMP-2 gene lead to decreases in BMP-2 promoter activity. Electrophoretic mobility shift assay using nuclear extracts from TMC-23 chondrocytic cells revealed that the NF-kappaB subunits p50 and p65 bound to the NF-kappaB response elements of the BMP-2 gene. Thus, NF-kappaB may positively regulate BMP-2 gene transcription. Consistent with these findings, expression of BMP-2 mRNA was significantly reduced in growth plate chondrocytes in NF-kappaB p50/p52 dKO mice, which associated with decreased numbers of 5-bromo-2'-deoxyuridine (BrdUrd)-positive cells in the proliferating zone of growth plate in these mice. Therefore, in postnatal growth plate chondrocytes, expression of BMP-2 is regulated by NF-kappaB, which may play an important role in chondrogenesis."xsd:string |
| http://purl.uniprot.org/citations/12759356 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m212296200"xsd:string |
| http://purl.uniprot.org/citations/12759356 | http://purl.uniprot.org/core/author | "Chen D."xsd:string |
| http://purl.uniprot.org/citations/12759356 | http://purl.uniprot.org/core/author | "Zhao M."xsd:string |
| http://purl.uniprot.org/citations/12759356 | http://purl.uniprot.org/core/author | "Xing L."xsd:string |
| http://purl.uniprot.org/citations/12759356 | http://purl.uniprot.org/core/author | "Horn D."xsd:string |
| http://purl.uniprot.org/citations/12759356 | http://purl.uniprot.org/core/author | "Chan J."xsd:string |
| http://purl.uniprot.org/citations/12759356 | http://purl.uniprot.org/core/author | "Zhang J.H."xsd:string |
| http://purl.uniprot.org/citations/12759356 | http://purl.uniprot.org/core/author | "Feng J.Q."xsd:string |
| http://purl.uniprot.org/citations/12759356 | http://purl.uniprot.org/core/author | "Harris S.E."xsd:string |
| http://purl.uniprot.org/citations/12759356 | http://purl.uniprot.org/core/author | "Mundy G.R."xsd:string |
| http://purl.uniprot.org/citations/12759356 | http://purl.uniprot.org/core/author | "Boyce B.F."xsd:string |
| http://purl.uniprot.org/citations/12759356 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12759356 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/12759356 | http://purl.uniprot.org/core/pages | "29130-29135"xsd:string |
| http://purl.uniprot.org/citations/12759356 | http://purl.uniprot.org/core/title | "NF-kappaB specifically activates BMP-2 gene expression in growth plate chondrocytes in vivo and in a chondrocyte cell line in vitro."xsd:string |
| http://purl.uniprot.org/citations/12759356 | http://purl.uniprot.org/core/volume | "278"xsd:string |
| http://purl.uniprot.org/citations/12759356 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12759356 |
| http://purl.uniprot.org/citations/12759356 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12759356 |
| http://purl.uniprot.org/uniprot/#_A0A0G2JGK6-mappedCitation-12759356 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12759356 |
| http://purl.uniprot.org/uniprot/#_A0A494B9Y5-mappedCitation-12759356 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12759356 |
| http://purl.uniprot.org/uniprot/#_A0A494BAQ2-mappedCitation-12759356 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12759356 |
| http://purl.uniprot.org/uniprot/#_F6Z9G5-mappedCitation-12759356 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12759356 |
| http://purl.uniprot.org/uniprot/#_P25799-mappedCitation-12759356 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12759356 |