| http://purl.uniprot.org/citations/12770499 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12770499 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12770499 | http://www.w3.org/2000/01/rdf-schema#comment | "Brevibacillus choshinensis (Bacillus brevis) is a protein-hyperproducing bacterium with a useful host-vector system for the production of recombinant proteins. Here, we cloned the ccdA-catA (cmacr;cdA āssociated thioredoxin-like tmacr;hiol-disulfide oxidoreductase) locus of B. choshinensis HPD31-S5. CatA protein (molecular weight, 19664) contains a thioredoxin-like motif, Cys-Gly-Pro-Cys. It was successfully expressed in B. choshinensis extracellularly ( approximately 100 microg x ml(-1) culture) using the secretion vector pNCMO2, and in Escherichia coli intracellularly ( approximately 350 microg x ml(-1) culture) with an amino-terminal His-tag. Both recombinant proteins showed thiol-disulfide oxidoreductase activity. Incubation of non-native human epidermal growth factor (hEGF) containing incorrect disulfide bonds with B. choshinensis cells secreting CatA protein resulted in the stimulation of the conversion of non-native hEGF to the native form. Furthermore, co-expression of CatA protein with recombinant hEGF in the B. choshinensis production system increased the yield of native hEGF."xsd:string |
| http://purl.uniprot.org/citations/12770499 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0168-1656(03)00071-3"xsd:string |
| http://purl.uniprot.org/citations/12770499 | http://purl.uniprot.org/core/author | "Ishibashi M."xsd:string |
| http://purl.uniprot.org/citations/12770499 | http://purl.uniprot.org/core/author | "Ishibashi M."xsd:string |
| http://purl.uniprot.org/citations/12770499 | http://purl.uniprot.org/core/author | "Mizukami M."xsd:string |
| http://purl.uniprot.org/citations/12770499 | http://purl.uniprot.org/core/author | "Mizukami M."xsd:string |
| http://purl.uniprot.org/citations/12770499 | http://purl.uniprot.org/core/author | "Tanaka R."xsd:string |
| http://purl.uniprot.org/citations/12770499 | http://purl.uniprot.org/core/author | "Tanaka R."xsd:string |
| http://purl.uniprot.org/citations/12770499 | http://purl.uniprot.org/core/author | "Tokunaga H."xsd:string |
| http://purl.uniprot.org/citations/12770499 | http://purl.uniprot.org/core/author | "Tokunaga H."xsd:string |
| http://purl.uniprot.org/citations/12770499 | http://purl.uniprot.org/core/author | "Tokunaga M."xsd:string |
| http://purl.uniprot.org/citations/12770499 | http://purl.uniprot.org/core/author | "Tokunaga M."xsd:string |
| http://purl.uniprot.org/citations/12770499 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12770499 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12770499 | http://purl.uniprot.org/core/name | "J. Biotechnol."xsd:string |
| http://purl.uniprot.org/citations/12770499 | http://purl.uniprot.org/core/name | "J Biotechnol"xsd:string |
| http://purl.uniprot.org/citations/12770499 | http://purl.uniprot.org/core/pages | "1-10"xsd:string |
| http://purl.uniprot.org/citations/12770499 | http://purl.uniprot.org/core/pages | "1-10"xsd:string |
| http://purl.uniprot.org/citations/12770499 | http://purl.uniprot.org/core/title | "Cloning and expression of the ccdA-associated thiol-disulfide oxidoreductase (catA) gene from Brevibacillus choshinensis: stimulation of human epidermal growth factor production."xsd:string |
| http://purl.uniprot.org/citations/12770499 | http://purl.uniprot.org/core/title | "Cloning and expression of the ccdA-associated thiol-disulfide oxidoreductase (catA) gene from Brevibacillus choshinensis: stimulation of human epidermal growth factor production."xsd:string |
| http://purl.uniprot.org/citations/12770499 | http://purl.uniprot.org/core/volume | "103"xsd:string |
| http://purl.uniprot.org/citations/12770499 | http://purl.uniprot.org/core/volume | "103"xsd:string |
| http://purl.uniprot.org/citations/12770499 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12770499 |