Results
Your Query
▶
▶
| Subject | Predicate | Object |
|---|---|---|
| http://purl.uniprot.org/citations/12777385 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12777385 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12777385 | http://www.w3.org/2000/01/rdf-schema#comment | "Nop56p is a component of the box C/D small nucleolar ribonucleoprotein complexes that direct 2'-O-methylation of pre-rRNA during its maturation. Genetic analyses in yeast have shown that Nop56p plays important roles in the early steps of pre-rRNA processing. However, its precise function remains elusive, especially in higher eukaryotes. Here we describe the proteomic characterization of human Nop56p (hNop56p)-associated pre-ribosomal ribonucleoprotein complexes. Mass spectrometric analysis of purified pre-ribosomal ribonucleoprotein complexes identified 61 ribosomal proteins, 16 trans-acting factors probably involved in ribosome biogenesis, and 29 proteins whose function in ribosome biogenesis is unknown. Identification of pre-rRNA species within hNop56p-associated pre-ribosomal ribonucleoprotein complexes, coupled with the known functions of yeast orthologs of the probable trans-acting factors identified in human, demonstrated that hNop56p functions in the early to middle stages of 60 S subunit synthesis in human cells. Interestingly, the nucleolar phosphoprotein treacle, which is responsible for the craniofacial disorder associated with Treacher Collins syndrome, was found to be a constituent of hNop56p-associated pre-rRNP complexes. The association of hNop56p and treacle within the complexes was independent of rRNA integrity, indicating a direct interaction. In addition, the protein compositions of the treacle-associated and hNop56p-associated pre-ribosomal ribonucleoprotein complexes were very similar, suggesting functional similarities between these two complexes with respect to ribosome biogenesis in human cells."xsd:string |
| http://purl.uniprot.org/citations/12777385 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m304304200"xsd:string |
| http://purl.uniprot.org/citations/12777385 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m304304200"xsd:string |
| http://purl.uniprot.org/citations/12777385 | http://purl.uniprot.org/core/author | "Isobe T."xsd:string |
| http://purl.uniprot.org/citations/12777385 | http://purl.uniprot.org/core/author | "Isobe T."xsd:string |
| http://purl.uniprot.org/citations/12777385 | http://purl.uniprot.org/core/author | "Yamauchi Y."xsd:string |
| http://purl.uniprot.org/citations/12777385 | http://purl.uniprot.org/core/author | "Yamauchi Y."xsd:string |
| http://purl.uniprot.org/citations/12777385 | http://purl.uniprot.org/core/author | "Takahashi N."xsd:string |
| http://purl.uniprot.org/citations/12777385 | http://purl.uniprot.org/core/author | "Takahashi N."xsd:string |
| http://purl.uniprot.org/citations/12777385 | http://purl.uniprot.org/core/author | "Shinkawa T."xsd:string |
| http://purl.uniprot.org/citations/12777385 | http://purl.uniprot.org/core/author | "Shinkawa T."xsd:string |
| http://purl.uniprot.org/citations/12777385 | http://purl.uniprot.org/core/author | "Yanagida M."xsd:string |
| http://purl.uniprot.org/citations/12777385 | http://purl.uniprot.org/core/author | "Yanagida M."xsd:string |
| http://purl.uniprot.org/citations/12777385 | http://purl.uniprot.org/core/author | "Hayano T."xsd:string |
| http://purl.uniprot.org/citations/12777385 | http://purl.uniprot.org/core/author | "Hayano T."xsd:string |
| http://purl.uniprot.org/citations/12777385 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12777385 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12777385 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12777385 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12777385 | http://purl.uniprot.org/core/pages | "34309-34319"xsd:string |
| http://purl.uniprot.org/citations/12777385 | http://purl.uniprot.org/core/pages | "34309-34319"xsd:string |
| http://purl.uniprot.org/citations/12777385 | http://purl.uniprot.org/core/title | "Proteomic analysis of human Nop56p-associated pre-ribosomal ribonucleoprotein complexes. Possible link between Nop56p and the nucleolar protein treacle responsible for Treacher Collins syndrome."xsd:string |
| http://purl.uniprot.org/citations/12777385 | http://purl.uniprot.org/core/title | "Proteomic analysis of human Nop56p-associated pre-ribosomal ribonucleoprotein complexes. Possible link between Nop56p and the nucleolar protein treacle responsible for Treacher Collins syndrome."xsd:string |