| http://purl.uniprot.org/citations/12782149 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12782149 | http://www.w3.org/2000/01/rdf-schema#comment | "The metabolism of 25-hydroxyvitamin D(3) was studied with a crude mitochondrial cytochrome P450 extract from pig kidney and with recombinant human CYP27A1 (mitochondrial vitamin D(3) 25-hydroxylase) and porcine CYP2D25 (microsomal vitamin D(3) 25-hydroxylase). The kidney mitochondrial cytochrome P450 catalyzed the formation of 1alpha,25-dihydroxyvitamin D(3), 24,25-dihydroxyvitamin D(3) and 25,27-dihydroxyvitamin D(3). An additional metabolite that was separated from the other hydroxylated products on HPLC was also formed. The formation of this 25-hydroxyvitamin D(3) metabolite was dependent on NADPH and the mitochondrial electron transferring protein components. A monoclonal antibody directed against purified pig liver CYP27A1 immunoprecipitated the 1alpha- and 27-hydroxylase activities towards 25-hydroxyvitamin D(3) as well as the formation of the unknown metabolite. These results together with substrate inhibition experiments indicate that CYP27A1 is responsible for the formation of the unknown 25-hydroxyvitamin D(3) metabolite in kidney. Recombinant human CYP27A1 was found to convert 25-hydroxyvitamin D(3) into 1alpha,25-dihydroxyvitamin D(3), 25,27-dihydroxyvitamin D(3) and a major metabolite with the same retention time on HPLC as that formed by kidney mitochondrial cytochrome P450. Gas chromatography-mass spectrometry (GC-MS) analysis of the unknown enzymatic product revealed it to be a triol different from other known hydroxylated 25-hydroxyvitamin D(3) metabolites such as 1alpha,25-, 23,25-, 24,25-, 25,26- or 25,27-dihydroxyvitamin D(3). The product had the mass spectrometic properties expected for 4beta,25-dihydroxyvitamin D(3). Recombinant porcine CYP2D25 converted 25-hydroxyvitamin D(3) into 1alpha,25-dihydroxyvitamin D(3) and 25,26-dihydroxyvitamin D(3). It can be concluded that both CYP27A1 and CYP2D25 are able to carry out multiple hydroxylations of 25-hydroxyvitamin D(3)."xsd:string |
| http://purl.uniprot.org/citations/12782149 | http://purl.org/dc/terms/identifier | "doi:10.1016/s1388-1981(03)00062-3"xsd:string |
| http://purl.uniprot.org/citations/12782149 | http://purl.uniprot.org/core/author | "Bodin K."xsd:string |
| http://purl.uniprot.org/citations/12782149 | http://purl.uniprot.org/core/author | "Araya Z."xsd:string |
| http://purl.uniprot.org/citations/12782149 | http://purl.uniprot.org/core/author | "Wikvall K."xsd:string |
| http://purl.uniprot.org/citations/12782149 | http://purl.uniprot.org/core/author | "Hosseinpour F."xsd:string |
| http://purl.uniprot.org/citations/12782149 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12782149 | http://purl.uniprot.org/core/name | "Biochim Biophys Acta"xsd:string |
| http://purl.uniprot.org/citations/12782149 | http://purl.uniprot.org/core/pages | "40-47"xsd:string |
| http://purl.uniprot.org/citations/12782149 | http://purl.uniprot.org/core/title | "Metabolism of 25-hydroxyvitamin D3 by microsomal and mitochondrial vitamin D3 25-hydroxylases (CYP2D25 and CYP27A1): a novel reaction by CYP27A1."xsd:string |
| http://purl.uniprot.org/citations/12782149 | http://purl.uniprot.org/core/volume | "1632"xsd:string |
| http://purl.uniprot.org/citations/12782149 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12782149 |
| http://purl.uniprot.org/citations/12782149 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12782149 |
| http://purl.uniprot.org/uniprot/#_A0A0C4DGN7-mappedCitation-12782149 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12782149 |
| http://purl.uniprot.org/uniprot/#_A0A0A0MSZ4-mappedCitation-12782149 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12782149 |
| http://purl.uniprot.org/uniprot/#_A0A0A0MT64-mappedCitation-12782149 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12782149 |
| http://purl.uniprot.org/uniprot/#_A0A0A0MTN9-mappedCitation-12782149 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12782149 |
| http://purl.uniprot.org/uniprot/#_A0A0A0MTR6-mappedCitation-12782149 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12782149 |
| http://purl.uniprot.org/uniprot/#_A0A0C4DFN8-mappedCitation-12782149 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12782149 |
| http://purl.uniprot.org/uniprot/#_B3KSQ6-mappedCitation-12782149 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12782149 |
| http://purl.uniprot.org/uniprot/#_B4DDI9-mappedCitation-12782149 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12782149 |
| http://purl.uniprot.org/uniprot/#_P10109-mappedCitation-12782149 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12782149 |
| http://purl.uniprot.org/uniprot/#_P22570-mappedCitation-12782149 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12782149 |
| http://purl.uniprot.org/uniprot/#_Q6GSK2-mappedCitation-12782149 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12782149 |