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| http://purl.uniprot.org/citations/12796494 | http://www.w3.org/2000/01/rdf-schema#comment | "We have reported that human immunodeficiency virus type 1 (HIV-1) integrase (IN) forms a specific nuclear complex with human lens epithelium-derived growth factor/transcription co-activator p75 (LEDGF/p75) protein. We now studied the IN-LEDGF/p75 interaction and nuclear import of IN in living cells using fusions of IN and LEDGF/p75 with enhanced green fluorescent protein and far-red fluorescent protein HcRed1. We show that both the N-terminal zinc binding domain and the central core domains of IN are involved in the interaction with LEDGF/p75. Both domains are essential for nuclear localization of IN as well as for the association of IN with condensed chromosomes during mitosis. However, upon overexpression of LEDGF/p75, the core domain fragment of IN was recruited to the nuclei and mitotic chromosomes with a distribution pattern characteristic of the full-length protein, indicating that it harbors the main determinant for interaction with LEDGF/p75. Although the C-terminal domain of IN was dispensable for nuclear/chromosomal localization, a fusion of the C-terminal IN fragment with enhanced green fluorescent protein was found exclusively in the nucleus, with a diffuse nuclear/nucleolar distribution, suggesting that the C-terminal domain may also play a role in the nuclear import of IN. In contrast to LEDGF/p75, its alternative splice variant, p52, did not interact with HIV-1 IN in vitro and in living cells. Finally, RNA interference-mediated knock-down of endogenous LEDGF/p75 expression abolished nuclear/chromosomal localization of IN. We conclude, therefore, that the interaction with LEDGF/p75 accounts for the karyophilic properties and chromosomal targeting of HIV-1 IN."xsd:string |
| http://purl.uniprot.org/citations/12796494 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m303594200"xsd:string |
| http://purl.uniprot.org/citations/12796494 | http://purl.uniprot.org/core/author | "Engelborghs Y."xsd:string |
| http://purl.uniprot.org/citations/12796494 | http://purl.uniprot.org/core/author | "De Clercq E."xsd:string |
| http://purl.uniprot.org/citations/12796494 | http://purl.uniprot.org/core/author | "Debyser Z."xsd:string |
| http://purl.uniprot.org/citations/12796494 | http://purl.uniprot.org/core/author | "Cherepanov P."xsd:string |
| http://purl.uniprot.org/citations/12796494 | http://purl.uniprot.org/core/author | "Busschots K."xsd:string |
| http://purl.uniprot.org/citations/12796494 | http://purl.uniprot.org/core/author | "Maertens G."xsd:string |
| http://purl.uniprot.org/citations/12796494 | http://purl.uniprot.org/core/author | "Pluymers W."xsd:string |
| http://purl.uniprot.org/citations/12796494 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12796494 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/12796494 | http://purl.uniprot.org/core/pages | "33528-33539"xsd:string |
| http://purl.uniprot.org/citations/12796494 | http://purl.uniprot.org/core/title | "LEDGF/p75 is essential for nuclear and chromosomal targeting of HIV-1 integrase in human cells."xsd:string |
| http://purl.uniprot.org/citations/12796494 | http://purl.uniprot.org/core/volume | "278"xsd:string |
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