| http://purl.uniprot.org/citations/1280830 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1280830 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1280830 | http://www.w3.org/2000/01/rdf-schema#comment | "We recently found that scatter factor (SF), a cell motility factor with a multimodular structure, is identical to hepatocyte growth factor (HGF), a potent mitogen of various cell types. SF/HGF is the ligand of the c-Met receptor tyrosine kinase. Here we used transient expression of naturally occurring and in vitro mutagenized cDNAs of SF/HGF to delineate the protein domains necessary for biological activity and binding to the c-Met receptor. (i) A single-chain SF/HGF resulting from the destruction of the protease cleavage site between heavy and light chain (Arg-494--> Gln) was largely inactive, indicating that proteolytic cleavage is essential for acquisition of the biologically active conformation. (ii) A SF/HGF splice variant encoding a protein with a 5-amino acid deletion in the first kringle domain was as highly active as the wild-type molecule. (iii) The separately expressed light chain (with serine protease homology) was inactive in all assays tested. (iv) The separate heavy chain as well as a naturally occurring splice variant consisting of the N terminus and the first two kringle domains bound the c-Met receptor, stimulated tyrosine auto-phosphorylation, and induced scattering of epithelial cells but not mitogenesis. These data indicate that a functional domain in the N terminus/first two kringle regions of SF/HGF is sufficient for binding to the Met receptor and that this leads to the activation of the downstream signal cascade involved in the motility response. However, the complete SF/HGF protein seems to be required for mitogenic activity."xsd:string |
| http://purl.uniprot.org/citations/1280830 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.89.23.11574"xsd:string |
| http://purl.uniprot.org/citations/1280830 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.89.23.11574"xsd:string |
| http://purl.uniprot.org/citations/1280830 | http://purl.uniprot.org/core/author | "Weidner K.M."xsd:string |
| http://purl.uniprot.org/citations/1280830 | http://purl.uniprot.org/core/author | "Weidner K.M."xsd:string |
| http://purl.uniprot.org/citations/1280830 | http://purl.uniprot.org/core/author | "Birchmeier W."xsd:string |
| http://purl.uniprot.org/citations/1280830 | http://purl.uniprot.org/core/author | "Birchmeier W."xsd:string |
| http://purl.uniprot.org/citations/1280830 | http://purl.uniprot.org/core/author | "Naldini L."xsd:string |
| http://purl.uniprot.org/citations/1280830 | http://purl.uniprot.org/core/author | "Naldini L."xsd:string |
| http://purl.uniprot.org/citations/1280830 | http://purl.uniprot.org/core/author | "Hartmann G."xsd:string |
| http://purl.uniprot.org/citations/1280830 | http://purl.uniprot.org/core/author | "Hartmann G."xsd:string |
| http://purl.uniprot.org/citations/1280830 | http://purl.uniprot.org/core/author | "Sachs M."xsd:string |
| http://purl.uniprot.org/citations/1280830 | http://purl.uniprot.org/core/author | "Sachs M."xsd:string |
| http://purl.uniprot.org/citations/1280830 | http://purl.uniprot.org/core/author | "Comoglio P.M."xsd:string |
| http://purl.uniprot.org/citations/1280830 | http://purl.uniprot.org/core/author | "Comoglio P.M."xsd:string |
| http://purl.uniprot.org/citations/1280830 | http://purl.uniprot.org/core/author | "Vigna E."xsd:string |
| http://purl.uniprot.org/citations/1280830 | http://purl.uniprot.org/core/author | "Vigna E."xsd:string |
| http://purl.uniprot.org/citations/1280830 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1280830 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1280830 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/1280830 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/1280830 | http://purl.uniprot.org/core/pages | "11574-11578"xsd:string |
| http://purl.uniprot.org/citations/1280830 | http://purl.uniprot.org/core/pages | "11574-11578"xsd:string |