| http://purl.uniprot.org/citations/12810533 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12810533 | http://www.w3.org/2000/01/rdf-schema#comment | "Both the amino-terminal and carboxyl-terminal domains of IGF binding protein (IGFBP)-3 are believed to contribute to high-affinity IGF binding. To investigate cooperativity in IGF binding by these domains, we expressed IGFBP-3 fragments 1-88 (NBP-3) and 185-264 (CBP-3) as FLAG and hexahistidine-tagged fusion proteins, respectively. IGF-I and IGF-II bound to NBP-3 poorly and to CBP-3 with moderate affinities, approximately 1 liter/nmol. Coincubating the fragments in equimolar concentrations caused a significant cooperative increase in IGF binding, demonstrated by immunoprecipitation with IGFBP-3, FLAG, or hexahistidine antibodies. Equimolar NBP-3 + CBP-3 bound IGF-II with an affinity (12.2 liter/nmol) only 4-fold lower than that of the IGFBP-3-IGF-II complex and IGF-I with an affinity (3.2 liter/nmol) 13-fold lower than IGFBP-3-IGF-I. Heterotrimeric complexes of NBP-3, CBP-3, and IGF, also demonstrated by affinity labeling, bound acid-labile subunit poorly. Coprecipitation assays with iodinated NBP-3 or CBP-3 indicated that the fragments cannot interact unless IGF is also present. Complexing with NBP-3 + CBP-3 inhibited IGF stimulation of type 1 IGF receptor activity and IGF-II binding to the type II receptor. This study demonstrates that isolated amino-terminal and carboxyl-terminal domains of IGFBP-3 cooperate in the presence of IGFs to form high-affinity complexes that retain the ability to block IGF activity."xsd:string |
| http://purl.uniprot.org/citations/12810533 | http://purl.org/dc/terms/identifier | "doi:10.1210/en.2003-0102"xsd:string |
| http://purl.uniprot.org/citations/12810533 | http://purl.uniprot.org/core/author | "Baxter R.C."xsd:string |
| http://purl.uniprot.org/citations/12810533 | http://purl.uniprot.org/core/author | "Wang X.H."xsd:string |
| http://purl.uniprot.org/citations/12810533 | http://purl.uniprot.org/core/author | "Firth S.M."xsd:string |
| http://purl.uniprot.org/citations/12810533 | http://purl.uniprot.org/core/author | "Payet L.D."xsd:string |
| http://purl.uniprot.org/citations/12810533 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12810533 | http://purl.uniprot.org/core/name | "Endocrinology"xsd:string |
| http://purl.uniprot.org/citations/12810533 | http://purl.uniprot.org/core/pages | "2797-2806"xsd:string |
| http://purl.uniprot.org/citations/12810533 | http://purl.uniprot.org/core/title | "Amino- and carboxyl-terminal fragments of insulin-like growth factor (IGF) binding protein-3 cooperate to bind IGFs with high affinity and inhibit IGF receptor interactions."xsd:string |
| http://purl.uniprot.org/citations/12810533 | http://purl.uniprot.org/core/volume | "144"xsd:string |
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