| http://purl.uniprot.org/citations/1281815 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1281815 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1281815 | http://www.w3.org/2000/01/rdf-schema#comment | "The glycoprotein gp210 is located in the "pore membrane," a specialized domain of the nuclear envelope to which the nuclear pore complex (NPC) is anchored. gp210 contains a large cisternal domain, a single transmembrane segment (TM), and a COOH-terminal, 58-amino acid residue cytoplasmic tail (CT) (Wozniak, R. W., E. Bartnik, and G. Blobel. 1989. J. Cell Biol. 108:2083-2092; Greber, U. F., A. Senior, and L. Gerace. 1990. EMBO (Eur. Mol. Biol. Organ.) J. 9:1495-1502). To locate determinants for sorting of gp210 to the pore membrane, we constructed various cDNAs coding for wild-type, mutant, and chimeric gp210, and monitored localization of the expressed protein in 3T3 cells by immunofluorescence microscopy using appropriate antibodies. The large cisternal domain of gp210 (95% of its mass) did not reveal any sorting determinants. Surprisingly, the TM of gp210 is sufficient for sorting to the pore membrane. The CT also contains a sorting determinant, but it is weaker than that of the TM. We propose specific lateral association of the transmembrane helices of two proteins to yield either a gp210 homodimer or a heterodimer of gp210 and another protein. The cytoplasmically oriented tails of these dimers may bind cooperatively to the adjacent NPCs. In addition, we demonstrate that gp210 co-localizes with cytoplasmically dispersed nucleoporins, suggesting a cytoplasmic association of these components."xsd:string |
| http://purl.uniprot.org/citations/1281815 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.119.6.1441"xsd:string |
| http://purl.uniprot.org/citations/1281815 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.119.6.1441"xsd:string |
| http://purl.uniprot.org/citations/1281815 | http://purl.uniprot.org/core/author | "Blobel G."xsd:string |
| http://purl.uniprot.org/citations/1281815 | http://purl.uniprot.org/core/author | "Blobel G."xsd:string |
| http://purl.uniprot.org/citations/1281815 | http://purl.uniprot.org/core/author | "Wozniak R.W."xsd:string |
| http://purl.uniprot.org/citations/1281815 | http://purl.uniprot.org/core/author | "Wozniak R.W."xsd:string |
| http://purl.uniprot.org/citations/1281815 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1281815 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1281815 | http://purl.uniprot.org/core/name | "J. Cell Biol."xsd:string |
| http://purl.uniprot.org/citations/1281815 | http://purl.uniprot.org/core/name | "J. Cell Biol."xsd:string |
| http://purl.uniprot.org/citations/1281815 | http://purl.uniprot.org/core/pages | "1441-1449"xsd:string |
| http://purl.uniprot.org/citations/1281815 | http://purl.uniprot.org/core/pages | "1441-1449"xsd:string |
| http://purl.uniprot.org/citations/1281815 | http://purl.uniprot.org/core/title | "The single transmembrane segment of gp210 is sufficient for sorting to the pore membrane domain of the nuclear envelope."xsd:string |
| http://purl.uniprot.org/citations/1281815 | http://purl.uniprot.org/core/title | "The single transmembrane segment of gp210 is sufficient for sorting to the pore membrane domain of the nuclear envelope."xsd:string |
| http://purl.uniprot.org/citations/1281815 | http://purl.uniprot.org/core/volume | "119"xsd:string |
| http://purl.uniprot.org/citations/1281815 | http://purl.uniprot.org/core/volume | "119"xsd:string |
| http://purl.uniprot.org/citations/1281815 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1281815 |
| http://purl.uniprot.org/citations/1281815 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1281815 |
| http://purl.uniprot.org/citations/1281815 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/1281815 |
| http://purl.uniprot.org/citations/1281815 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/1281815 |
| http://purl.uniprot.org/uniprot/P11654 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/1281815 |
| http://purl.uniprot.org/uniprot/P11654#attribution-6AC32B56D67685E87CC0F50311A1722B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/1281815 |