| http://purl.uniprot.org/citations/12821939 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12821939 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12821939 | http://www.w3.org/2000/01/rdf-schema#comment | "Transformation of chick embryo fibroblasts by the v-Jun oncoprotein correlates with a downregulation of the extracellular matrix protein SPARC and repression of the corresponding mRNA. Repression of SPARC contributes to the oncogenic process by facilitating tumor development in vivo. A proximal promoter fragment, designated -124/+16, is responsible for high constitutive activity of the SPARC gene and is the target of repression by v-Jun. In this paper, using electrophoretic mobility shift and pull-down assays in vitro, and transient transfections and chromatin immunoprecipitation assays in Sp1/3-deficient Drosophila SL2 cells and in chick embryo fibroblasts, we show that (i) Sp1 and/or Sp3 is required for constitutive activation of SPARC transcription, by binding directly to the GGA-rich -92/-57 fragment; and (ii) v-Jun does not bind -124/+16 directly, but binds to the GGA-rich fragment indirectly, most likely through a physical interaction with Sp1/3. Moreover, a transactivation-proficient v-Jun derivative, designated v-Jun/cebp/glz, which cannot bind Jun DNA motifs anymore and cannot heterodimerize, is still capable of downregulating SPARC efficiently. Taken together, these data strongly suggest that v-Jun downregulates SPARC through the formation of a DNA-Sp1/3-v-Jun, chromatin-associated complex."xsd:string |
| http://purl.uniprot.org/citations/12821939 | http://purl.org/dc/terms/identifier | "doi:10.1038/sj.onc.1206713"xsd:string |
| http://purl.uniprot.org/citations/12821939 | http://purl.org/dc/terms/identifier | "doi:10.1038/sj.onc.1206713"xsd:string |
| http://purl.uniprot.org/citations/12821939 | http://purl.uniprot.org/core/author | "Briggs J."xsd:string |
| http://purl.uniprot.org/citations/12821939 | http://purl.uniprot.org/core/author | "Briggs J."xsd:string |
| http://purl.uniprot.org/citations/12821939 | http://purl.uniprot.org/core/author | "Castellazzi M."xsd:string |
| http://purl.uniprot.org/citations/12821939 | http://purl.uniprot.org/core/author | "Castellazzi M."xsd:string |
| http://purl.uniprot.org/citations/12821939 | http://purl.uniprot.org/core/author | "Galvagni F."xsd:string |
| http://purl.uniprot.org/citations/12821939 | http://purl.uniprot.org/core/author | "Galvagni F."xsd:string |
| http://purl.uniprot.org/citations/12821939 | http://purl.uniprot.org/core/author | "Oliviero S."xsd:string |
| http://purl.uniprot.org/citations/12821939 | http://purl.uniprot.org/core/author | "Oliviero S."xsd:string |
| http://purl.uniprot.org/citations/12821939 | http://purl.uniprot.org/core/author | "Bos T."xsd:string |
| http://purl.uniprot.org/citations/12821939 | http://purl.uniprot.org/core/author | "Bos T."xsd:string |
| http://purl.uniprot.org/citations/12821939 | http://purl.uniprot.org/core/author | "Chamboredon S."xsd:string |
| http://purl.uniprot.org/citations/12821939 | http://purl.uniprot.org/core/author | "Chamboredon S."xsd:string |
| http://purl.uniprot.org/citations/12821939 | http://purl.uniprot.org/core/author | "Hurault J."xsd:string |
| http://purl.uniprot.org/citations/12821939 | http://purl.uniprot.org/core/author | "Hurault J."xsd:string |
| http://purl.uniprot.org/citations/12821939 | http://purl.uniprot.org/core/author | "Vial E."xsd:string |
| http://purl.uniprot.org/citations/12821939 | http://purl.uniprot.org/core/author | "Vial E."xsd:string |
| http://purl.uniprot.org/citations/12821939 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12821939 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12821939 | http://purl.uniprot.org/core/name | "Oncogene"xsd:string |
| http://purl.uniprot.org/citations/12821939 | http://purl.uniprot.org/core/name | "Oncogene"xsd:string |