| http://purl.uniprot.org/citations/12832069 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12832069 | http://www.w3.org/2000/01/rdf-schema#comment | "Annexin A9 is a novel member of the annexin family of Ca(2+) and phospholipid binding proteins which has so far only been identified in EST data bases and whose deduced protein sequence shows mutations in residues considered crucial for Ca(2+) coordination in other annexins. To elucidate whether the annexin A9 protein is expressed as such and to characterize its biochemical properties we probed cell extracts with specific anti-annexin A9 antibodies and developed a recombinant expression system. We show that the protein is found in HepG2 hepatoma cell lysates and that a green fluorescent protein-tagged form is abundantly expressed in the cytosol of HeLa cells. Recombinant expression in bacteria yields a soluble protein that can be enriched by conventional chromatographic procedures. The protein is capable of binding phosphatidylserine containing liposomes albeit only at Ca(2+) concentrations exceeding 2 mM. Moreover and in contrast to other annexins this binding appears to be irreversible as the liposome-bound annexin A9 cannot be released by Ca(2+) chelation. These results indicate that annexin A9 is a unique member of the annexin family whose intracellular activity is not subject to Ca(2+) regulation."xsd:string |
| http://purl.uniprot.org/citations/12832069 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0014-5793(03)00634-3"xsd:string |
| http://purl.uniprot.org/citations/12832069 | http://purl.uniprot.org/core/author | "Gerke V."xsd:string |
| http://purl.uniprot.org/citations/12832069 | http://purl.uniprot.org/core/author | "Goebeler V."xsd:string |
| http://purl.uniprot.org/citations/12832069 | http://purl.uniprot.org/core/author | "Rescher U."xsd:string |
| http://purl.uniprot.org/citations/12832069 | http://purl.uniprot.org/core/author | "Ruhe D."xsd:string |
| http://purl.uniprot.org/citations/12832069 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12832069 | http://purl.uniprot.org/core/name | "FEBS Lett"xsd:string |
| http://purl.uniprot.org/citations/12832069 | http://purl.uniprot.org/core/pages | "359-364"xsd:string |
| http://purl.uniprot.org/citations/12832069 | http://purl.uniprot.org/core/title | "Atypical properties displayed by annexin A9, a novel member of the annexin family of Ca(2+) and lipid binding proteins."xsd:string |
| http://purl.uniprot.org/citations/12832069 | http://purl.uniprot.org/core/volume | "546"xsd:string |
| http://purl.uniprot.org/citations/12832069 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12832069 |
| http://purl.uniprot.org/citations/12832069 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12832069 |
| http://purl.uniprot.org/uniprot/O76027#attribution-87FE5E25C5AC3ED232501422C7622C07 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/12832069 |
| http://purl.uniprot.org/uniprot/#_O76027-mappedCitation-12832069 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12832069 |
| http://purl.uniprot.org/uniprot/O76027 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12832069 |