| http://purl.uniprot.org/citations/12832468 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12832468 | http://www.w3.org/2000/01/rdf-schema#comment | "Nonsense-mediated mRNA decay (NMD) is an RNA surveillance pathway that detects and destroys aberrant mRNAs containing nonsense or premature termination codons (PTCs) in a translation-dependent manner in eukaryotes. In yeast, the NMD pathway bypasses the deadenylation step and directly targets PTC-containing messages for decapping, followed by 5'-to-3' exonuclease digestion of the RNA body. In mammals, most PTC-containing mRNAs are subject to active nucleus-associated NMD. Here, using two distinct transcription-pulsing approaches to monitor mRNA deadenylation and decay kinetics, we demonstrate the existence of an active cytoplasmic NMD pathway in mammalian cells. In this pathway, a nonsense codon triggers accelerated deadenylation that precedes decay of the PTC-containing mRNA body. Transcript is stabilized when accelerated deadenylation is impeded by blocking translation initiation; by ectopically expressing two RNA-binding proteins, UNR and NSAP1; or by ectopically expressing a UPF1 dominant-negative mutant. These results are consistent with the notion that the nonsense codon can function in the cytoplasm by promoting rapid removal of the poly(A) tail as a necessary first step in the decay process."xsd:string |
| http://purl.uniprot.org/citations/12832468 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.23.14.4805-4813.2003"xsd:string |
| http://purl.uniprot.org/citations/12832468 | http://purl.uniprot.org/core/author | "Chen C.Y."xsd:string |
| http://purl.uniprot.org/citations/12832468 | http://purl.uniprot.org/core/author | "Shyu A.B."xsd:string |
| http://purl.uniprot.org/citations/12832468 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12832468 | http://purl.uniprot.org/core/name | "Mol Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/12832468 | http://purl.uniprot.org/core/pages | "4805-4813"xsd:string |
| http://purl.uniprot.org/citations/12832468 | http://purl.uniprot.org/core/title | "Rapid deadenylation triggered by a nonsense codon precedes decay of the RNA body in a mammalian cytoplasmic nonsense-mediated decay pathway."xsd:string |
| http://purl.uniprot.org/citations/12832468 | http://purl.uniprot.org/core/volume | "23"xsd:string |
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