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| http://purl.uniprot.org/citations/12834298 | http://www.w3.org/2000/01/rdf-schema#comment | "Aspergillus niger ATCC 9642 isopullulanase (IPU) was heterologously expressed by Pichia pastoris GS115 under three different signal sequences of Saccharomyces cerevisiae acid phosphatase, S. cerevisiae alpha-factor prepro peptide, and A. niger isopullulanase. One-step purification using lectin Con A affinity chromatography yielded recombinant IPU (IPU-PP) with high purity. IPU-PP had a higher carbohydrate content than native IPU and IPU-AO expressed in A. oryzae M-2-3. IPU-PP hydrolyzed various substrates containing the structure of panose, which indicated a strict subsite recognition of the panose motif."xsd:string |
| http://purl.uniprot.org/citations/12834298 | http://purl.org/dc/terms/identifier | "doi:10.1271/bbb.67.1149"xsd:string |
| http://purl.uniprot.org/citations/12834298 | http://purl.uniprot.org/core/author | "Aoki H."xsd:string |
| http://purl.uniprot.org/citations/12834298 | http://purl.uniprot.org/core/author | "Nishikawa A."xsd:string |
| http://purl.uniprot.org/citations/12834298 | http://purl.uniprot.org/core/author | "Kashiwagi Y."xsd:string |
| http://purl.uniprot.org/citations/12834298 | http://purl.uniprot.org/core/author | "Tonozuka T."xsd:string |
| http://purl.uniprot.org/citations/12834298 | http://purl.uniprot.org/core/author | "Sakano Y."xsd:string |
| http://purl.uniprot.org/citations/12834298 | http://purl.uniprot.org/core/author | "Akeboshi H."xsd:string |
| http://purl.uniprot.org/citations/12834298 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12834298 | http://purl.uniprot.org/core/name | "Biosci Biotechnol Biochem"xsd:string |
| http://purl.uniprot.org/citations/12834298 | http://purl.uniprot.org/core/pages | "1149-1153"xsd:string |
| http://purl.uniprot.org/citations/12834298 | http://purl.uniprot.org/core/title | "Construction of an efficient expression system for Aspergillus isopullulanase in Pichia pastoris, and a simple purification method."xsd:string |
| http://purl.uniprot.org/citations/12834298 | http://purl.uniprot.org/core/volume | "67"xsd:string |
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| http://purl.uniprot.org/uniprot/O00105 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12834298 |