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| http://purl.uniprot.org/citations/12838502 | http://www.w3.org/2000/01/rdf-schema#comment | "We have studied modulation of purinergic receptors (P(2Y) and P(2X) subtypes) on changes in intracellular Ca(2+) [Ca(2+)](i) and expression and production of COX-2 in human microglia. Measurements using Ca(2+)-sensitive spectrofluorometry showed adenosine triphosphate (ATP) to cause rapid transient increases in [Ca(2+)](i). Application of ATP plus the P(2X) antagonist, pyridoxal-phosphate-6-azophenyl-2',4'-disulfonic acid (PPADS), or treatment with adenosine diphosphate-beta-S (ADP-beta-S), a selective P(2Y) agonist, led to a considerable prolongation in [Ca(2+)](i) responses compared with ATP. The prolonged time courses were consistent with sustained activation of store-operated channels (SOC) since SKF96365, an inhibitor of SOC, blocked this component of the response. RT-PCR data showed that microglia expressed no COX-2 either constitutively or following treatment of cells with ATP (100 microM for 8 h). However, treatment using ATP plus PPADS or with ADP-beta-S led to marked expression of COX-2. The enhanced COX-2 with ATP plus PPADS treatment was absent in the presence of SKF96365 or using Ca(2+)-free solution. Immunocytochemistry, using a specific anti-COX-2 antibody, also revealed a pattern of purinergic modulation whereby lack of P(2X) activation enhanced the production of COX-2 protein. These results suggest that modulation of subtypes of purinergic receptors regulates COX-2 in human microglia with a link involving SOC-mediated influx of Ca(2+)."xsd:string |
| http://purl.uniprot.org/citations/12838502 | http://purl.org/dc/terms/identifier | "doi:10.1002/glia.10239"xsd:string |
| http://purl.uniprot.org/citations/12838502 | http://purl.uniprot.org/core/author | "Hong S.H."xsd:string |
| http://purl.uniprot.org/citations/12838502 | http://purl.uniprot.org/core/author | "Kim S.U."xsd:string |
| http://purl.uniprot.org/citations/12838502 | http://purl.uniprot.org/core/author | "Choi H.B."xsd:string |
| http://purl.uniprot.org/citations/12838502 | http://purl.uniprot.org/core/author | "Ryu J.K."xsd:string |
| http://purl.uniprot.org/citations/12838502 | http://purl.uniprot.org/core/author | "McLarnon J.G."xsd:string |
| http://purl.uniprot.org/citations/12838502 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12838502 | http://purl.uniprot.org/core/name | "Glia"xsd:string |
| http://purl.uniprot.org/citations/12838502 | http://purl.uniprot.org/core/pages | "95-103"xsd:string |
| http://purl.uniprot.org/citations/12838502 | http://purl.uniprot.org/core/title | "Differential activation of subtype purinergic receptors modulates Ca(2+) mobilization and COX-2 in human microglia."xsd:string |
| http://purl.uniprot.org/citations/12838502 | http://purl.uniprot.org/core/volume | "43"xsd:string |
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