| http://purl.uniprot.org/citations/12842885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12842885 | http://www.w3.org/2000/01/rdf-schema#comment | "In mammalian cells, membrane-bound sterol regulatory element-binding proteins (SREBPs) are transported from ER to Golgi where they are processed proteolytically to generate soluble transcription factors that activate lipid synthesis. ER-to-Golgi transport requires SCAP, a sterol-regulated escort protein. In sterol-treated cells, the SCAP/SREBP complex binds to Insig-1 or Insig-2, which retains the complex in the ER, blocking SREBP processing and decreasing lipid synthesis. In Drosophila cells, the endogenous SCAP/SREBP complex is transported to Golgi, but transport is blocked by phosphatidylethanolamine instead of sterols. Here, we show that mammalian SREBP-2 is not transported to Golgi when expressed in Drosophila cells. Transport requires co-expression of mammalian SCAP. Sterols block transport of the mammalian SCAP/SREBP-2 complex, but only when mammalian Insig-1 or -2 is co-expressed. These reconstitution studies define SCAP and Insig as the minimal requirements for sterol-regulated transport of SREBPs from ER to Golgi. They indicate that insect cells can respond to sterols when proper regulatory proteins are expressed."xsd:string |
| http://purl.uniprot.org/citations/12842885 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m306476200"xsd:string |
| http://purl.uniprot.org/citations/12842885 | http://purl.uniprot.org/core/author | "Brown M.S."xsd:string |
| http://purl.uniprot.org/citations/12842885 | http://purl.uniprot.org/core/author | "Goldstein J.L."xsd:string |
| http://purl.uniprot.org/citations/12842885 | http://purl.uniprot.org/core/author | "Dobrosotskaya I.Y."xsd:string |
| http://purl.uniprot.org/citations/12842885 | http://purl.uniprot.org/core/author | "Rawson R.B."xsd:string |
| http://purl.uniprot.org/citations/12842885 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12842885 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/12842885 | http://purl.uniprot.org/core/pages | "35837-35843"xsd:string |
| http://purl.uniprot.org/citations/12842885 | http://purl.uniprot.org/core/title | "Reconstitution of sterol-regulated endoplasmic reticulum-to-Golgi transport of SREBP-2 in insect cells by co-expression of mammalian SCAP and Insigs."xsd:string |
| http://purl.uniprot.org/citations/12842885 | http://purl.uniprot.org/core/volume | "278"xsd:string |
| http://purl.uniprot.org/citations/12842885 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12842885 |
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| http://purl.uniprot.org/uniprot/#_P53992-mappedCitation-12842885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12842885 |
| http://purl.uniprot.org/uniprot/#_B4DSU0-mappedCitation-12842885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12842885 |
| http://purl.uniprot.org/uniprot/#_B7Z6H0-mappedCitation-12842885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12842885 |
| http://purl.uniprot.org/uniprot/#_A1Z6J2-mappedCitation-12842885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12842885 |
| http://purl.uniprot.org/uniprot/#_B4DQ23-mappedCitation-12842885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12842885 |
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| http://purl.uniprot.org/uniprot/#_G3V0I8-mappedCitation-12842885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12842885 |
| http://purl.uniprot.org/uniprot/#_Q15436-mappedCitation-12842885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12842885 |
| http://purl.uniprot.org/uniprot/#_O95486-mappedCitation-12842885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12842885 |
| http://purl.uniprot.org/uniprot/#_O95487-mappedCitation-12842885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12842885 |
| http://purl.uniprot.org/uniprot/#_Q24146-mappedCitation-12842885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12842885 |