| http://purl.uniprot.org/citations/12847223 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12847223 | http://www.w3.org/2000/01/rdf-schema#comment | "We recently described the cloning of murine triggering receptor expressed by myeloid cells (TREM) 2, a single Ig domain DNAX adaptor protein 12-associated receptor expressed by cells of the myeloid lineage. In this study, we describe the identification of ligands for TREM-2 on both bacteria and mammalian cells. First, by using a TREM-2A/IgG1-Fc fusion protein, we demonstrate specific binding to a number of Gram-negative and Gram-positive bacteria and to yeast. Furthermore, we show that fluorescently labeled Escherichia coli and Staphylococcus aureus bind specifically to TREM-2-transfected cells. The binding of TREM-2A/Ig fusion protein to E. coli can be inhibited by the bacterial products LPS, lipoteichoic acid, and peptidoglycan. Additionally, binding can be inhibited by a number of other anionic carbohydrate molecules, including dextran sulfate, suggesting that ligand recognition is based partly on charge. Using a sensitive reporter assay, we demonstrate activation of a TREM-2A/CD3zeta chimeric receptor by both bacteria and dextran sulfate. Finally, we demonstrate binding of TREM-2A/Ig fusion to a series of human astrocytoma lines but not to a variety of other cell lines. The binding to astrocytomas, like binding to bacteria, is inhibited by anionic bacterial products, suggesting either a similar charge-based ligand recognition method or overlapping binding sites for recognition of self- and pathogen-expressed ligands."xsd:string |
| http://purl.uniprot.org/citations/12847223 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.171.2.594"xsd:string |
| http://purl.uniprot.org/citations/12847223 | http://purl.uniprot.org/core/author | "Sullam P.M."xsd:string |
| http://purl.uniprot.org/citations/12847223 | http://purl.uniprot.org/core/author | "Chen T.T."xsd:string |
| http://purl.uniprot.org/citations/12847223 | http://purl.uniprot.org/core/author | "Niemi E.C."xsd:string |
| http://purl.uniprot.org/citations/12847223 | http://purl.uniprot.org/core/author | "Daws M.R."xsd:string |
| http://purl.uniprot.org/citations/12847223 | http://purl.uniprot.org/core/author | "Seaman W.E."xsd:string |
| http://purl.uniprot.org/citations/12847223 | http://purl.uniprot.org/core/author | "Tchao N.K."xsd:string |
| http://purl.uniprot.org/citations/12847223 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12847223 | http://purl.uniprot.org/core/name | "J Immunol"xsd:string |
| http://purl.uniprot.org/citations/12847223 | http://purl.uniprot.org/core/pages | "594-599"xsd:string |
| http://purl.uniprot.org/citations/12847223 | http://purl.uniprot.org/core/title | "Pattern recognition by TREM-2: binding of anionic ligands."xsd:string |
| http://purl.uniprot.org/citations/12847223 | http://purl.uniprot.org/core/volume | "171"xsd:string |
| http://purl.uniprot.org/citations/12847223 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12847223 |
| http://purl.uniprot.org/citations/12847223 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12847223 |
| http://purl.uniprot.org/uniprot/Q99NH8#attribution-59EE6263B71A9B9A55F658FEBB43C2DD | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/12847223 |
| http://purl.uniprot.org/uniprot/Q99NH8#attribution-96AA9135342DC00CF4DA50A06907E3C3 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/12847223 |
| http://purl.uniprot.org/uniprot/#_Q3U2Q4-mappedCitation-12847223 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12847223 |
| http://purl.uniprot.org/uniprot/#_Q9JKE1-mappedCitation-12847223 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12847223 |
| http://purl.uniprot.org/uniprot/#_Q99NH8-mappedCitation-12847223 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12847223 |
| http://purl.uniprot.org/uniprot/Q9JKE1 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12847223 |
| http://purl.uniprot.org/uniprot/Q3U2Q4 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12847223 |
| http://purl.uniprot.org/uniprot/Q99NH8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12847223 |