| http://purl.uniprot.org/citations/12847250 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12847250 | http://www.w3.org/2000/01/rdf-schema#comment | "Previous studies have revealed that LPS can activate transcription of the IL-10 gene promoter through an SV40 promoter factor 1 (Sp1) binding site in mouse macrophage RAW264.7. In this study, we determined that, in addition to Sp1, C/EBPbeta and delta were also involved in LPS-induced gene expression of IL-10. By transient transfection with 5'-deletion mutants of the IL-10 promoter, we found that there were two LPS-responsive elements in the promoter of the mouse IL-10 gene. Analysis of these two regions by gel shift assay suggested that Sp1 and C/EBPbeta and delta were bound to these two regions, respectively. By site-directed mutagenesis, we found that disruption at both the Sp1 and C/EBP binding sites almost completely blocked the LPS response. By gel shift assay and Western blotting, we found that the DNA binding complex and protein expression of C/EBPbeta and delta were increased by LPS treatment, but these results were not found for Sp1. Overexpression of C/EBPbeta or C/EBPdelta, respectively, activated the promoter of the IL-10 gene, and they were enhanced by LPS. Coimmunoprecipitation experiments in intact cells indicated that LPS stimulated interaction between Sp1 and C/EBPbeta and delta. These results suggested that the interaction between Sp1 and C/EBPbeta and delta induced by LPS cooperatively activated expression of the IL-10 gene. The increase of C/EBPbeta and delta proteins and the enhancement of transactivation activity of C/EBPbeta and delta by LPS treatment, at least in part, explain the activation of IL-10 gene expression."xsd:string |
| http://purl.uniprot.org/citations/12847250 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.171.2.821"xsd:string |
| http://purl.uniprot.org/citations/12847250 | http://purl.uniprot.org/core/author | "Chang W.C."xsd:string |
| http://purl.uniprot.org/citations/12847250 | http://purl.uniprot.org/core/author | "Chen L.C."xsd:string |
| http://purl.uniprot.org/citations/12847250 | http://purl.uniprot.org/core/author | "Chen B.K."xsd:string |
| http://purl.uniprot.org/citations/12847250 | http://purl.uniprot.org/core/author | "Liu Y.W."xsd:string |
| http://purl.uniprot.org/citations/12847250 | http://purl.uniprot.org/core/author | "Tseng H.P."xsd:string |
| http://purl.uniprot.org/citations/12847250 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12847250 | http://purl.uniprot.org/core/name | "J Immunol"xsd:string |
| http://purl.uniprot.org/citations/12847250 | http://purl.uniprot.org/core/pages | "821-828"xsd:string |
| http://purl.uniprot.org/citations/12847250 | http://purl.uniprot.org/core/title | "Functional cooperation of simian virus 40 promoter factor 1 and CCAAT/enhancer-binding protein beta and delta in lipopolysaccharide-induced gene activation of IL-10 in mouse macrophages."xsd:string |
| http://purl.uniprot.org/citations/12847250 | http://purl.uniprot.org/core/volume | "171"xsd:string |
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