| http://purl.uniprot.org/citations/12853575 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12853575 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12853575 | http://www.w3.org/2000/01/rdf-schema#comment | "SNARE [soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor] proteins are essential for membrane fusion but their regulation is not yet fully understood. We have previously shown that the amino-terminal Longin domain of the v-SNARE TI-VAMP (tetanus neurotoxin-insensitive vesicle-associated membrane protein)/VAMP7 plays an inhibitory role in neurite outgrowth. The goal of this study was to investigate the regulation of TI-VAMP as a model of v-SNARE regulation. We show here that the Longin domain (LD) plays a dual role. First, it negatively regulates the ability of TI-VAMP and of a Longin/Synaptobrevin chimera to participate in SNARE complexes. Second, it interacts with the adaptor complex AP-3 and this interaction targets TI-VAMP to late endosomes. Accordingly, in mocha cells lacking AP-3 delta, TI-VAMP is retained in an early endosomal compartment. Furthermore, TI-VAMPc, an isoform of TI-VAMP lacking part of the LD, does not interact with AP-3, and therefore is not targeted to late endosomes; however, this shorter LD still inhibits SNARE-complex formation. These findings support a mechanism controlling both localization and function of TI-VAMP through the LD and clathrin adaptors. Moreover, they point to the amino-terminal domains of SNARE proteins as multifunctional modules responsible for the fine tuning of SNARE function."xsd:string |
| http://purl.uniprot.org/citations/12853575 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1431910100"xsd:string |
| http://purl.uniprot.org/citations/12853575 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1431910100"xsd:string |
| http://purl.uniprot.org/citations/12853575 | http://purl.uniprot.org/core/author | "Camonis J."xsd:string |
| http://purl.uniprot.org/citations/12853575 | http://purl.uniprot.org/core/author | "Camonis J."xsd:string |
| http://purl.uniprot.org/citations/12853575 | http://purl.uniprot.org/core/author | "Formstecher E."xsd:string |
| http://purl.uniprot.org/citations/12853575 | http://purl.uniprot.org/core/author | "Formstecher E."xsd:string |
| http://purl.uniprot.org/citations/12853575 | http://purl.uniprot.org/core/author | "Daviet L."xsd:string |
| http://purl.uniprot.org/citations/12853575 | http://purl.uniprot.org/core/author | "Daviet L."xsd:string |
| http://purl.uniprot.org/citations/12853575 | http://purl.uniprot.org/core/author | "Galli T."xsd:string |
| http://purl.uniprot.org/citations/12853575 | http://purl.uniprot.org/core/author | "Galli T."xsd:string |
| http://purl.uniprot.org/citations/12853575 | http://purl.uniprot.org/core/author | "D'Esposito M."xsd:string |
| http://purl.uniprot.org/citations/12853575 | http://purl.uniprot.org/core/author | "D'Esposito M."xsd:string |
| http://purl.uniprot.org/citations/12853575 | http://purl.uniprot.org/core/author | "Filippini F."xsd:string |
| http://purl.uniprot.org/citations/12853575 | http://purl.uniprot.org/core/author | "Filippini F."xsd:string |
| http://purl.uniprot.org/citations/12853575 | http://purl.uniprot.org/core/author | "Vacca M."xsd:string |
| http://purl.uniprot.org/citations/12853575 | http://purl.uniprot.org/core/author | "Vacca M."xsd:string |
| http://purl.uniprot.org/citations/12853575 | http://purl.uniprot.org/core/author | "Hamburger A."xsd:string |
| http://purl.uniprot.org/citations/12853575 | http://purl.uniprot.org/core/author | "Hamburger A."xsd:string |
| http://purl.uniprot.org/citations/12853575 | http://purl.uniprot.org/core/author | "Martinez-Arca S."xsd:string |
| http://purl.uniprot.org/citations/12853575 | http://purl.uniprot.org/core/author | "Martinez-Arca S."xsd:string |
| http://purl.uniprot.org/citations/12853575 | http://purl.uniprot.org/core/author | "Rudge R."xsd:string |
| http://purl.uniprot.org/citations/12853575 | http://purl.uniprot.org/core/author | "Rudge R."xsd:string |