| http://purl.uniprot.org/citations/12855688 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12855688 | http://www.w3.org/2000/01/rdf-schema#comment | "To provide insight into the physiological importance of 3-phosphoinositide-dependent kinase-1 (PDK-1) in the metabolic actions of insulin, we have generated mice that harbor a PDK-1 gene containing LoxP sites (PDK-1(lox/lox) mice) and established immortalized brown preadipocyte cell lines both from these animals and from wild-type mice. Exposure to appropriate hormonal inducers resulted in the differentiation of >80% of the immortalized brown preadipocytes derived from both types of mice into mature adipocytes. Introduction of the Cre recombinase with the use of adenovirus-mediated gene transfer induced a dose-dependent decrease in the abundance of PDK-1 in PDK-1(lox/lox) adipocytes but not in the wild-type cells. In Cre-expressing PDK-1(lox/lox) adipocytes in which the abundance of PDK-1 was reduced by approximately 85%, the insulin-induced phosphorylation both of Akt on threonine 308 and of p70 S6 kinase on threonine-389 was markedly inhibited. The phosphorylation both of Akt on serine 473 and of p42 and p44 isoforms of mitogen-activated protein kinase induced by insulin was not affected by Cre expression, indicating that the latter specifically inhibits PDK-1-dependent signaling. Both glucose uptake and the translocation of glucose transporter 4 to the plasma membrane induced by insulin as well as glucose uptake induced by a constitutively active form of phosphoinositide 3-kinase were also greatly inhibited by Cre expression in PDK-1(lox/lox) adipocytes. Phosphorylation of AMP-activated protein kinase and glucose uptake induced by 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) were not affected by Cre expression in PDK-1(lox/lox) adipocytes. These results indicate that PDK-1 is essential for insulin-induced glucose uptake in adipocytes."xsd:string |
| http://purl.uniprot.org/citations/12855688 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m306151200"xsd:string |
| http://purl.uniprot.org/citations/12855688 | http://purl.uniprot.org/core/author | "Kasuga M."xsd:string |
| http://purl.uniprot.org/citations/12855688 | http://purl.uniprot.org/core/author | "Mori T."xsd:string |
| http://purl.uniprot.org/citations/12855688 | http://purl.uniprot.org/core/author | "Noda T."xsd:string |
| http://purl.uniprot.org/citations/12855688 | http://purl.uniprot.org/core/author | "Ogawa W."xsd:string |
| http://purl.uniprot.org/citations/12855688 | http://purl.uniprot.org/core/author | "Sakaue H."xsd:string |
| http://purl.uniprot.org/citations/12855688 | http://purl.uniprot.org/core/author | "Nishizawa A."xsd:string |
| http://purl.uniprot.org/citations/12855688 | http://purl.uniprot.org/core/author | "Takashima Y."xsd:string |
| http://purl.uniprot.org/citations/12855688 | http://purl.uniprot.org/core/author | "Teshigawara K."xsd:string |
| http://purl.uniprot.org/citations/12855688 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12855688 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/12855688 | http://purl.uniprot.org/core/pages | "38870-38874"xsd:string |
| http://purl.uniprot.org/citations/12855688 | http://purl.uniprot.org/core/title | "Requirement for 3-phosphoinositide-kependent dinase-1 (PDK-1) in insulin-induced glucose uptake in immortalized brown adipocytes."xsd:string |
| http://purl.uniprot.org/citations/12855688 | http://purl.uniprot.org/core/volume | "278"xsd:string |
| http://purl.uniprot.org/citations/12855688 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12855688 |
| http://purl.uniprot.org/citations/12855688 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12855688 |
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| http://purl.uniprot.org/uniprot/#_F2Z3X6-mappedCitation-12855688 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12855688 |
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| http://purl.uniprot.org/uniprot/#_F2Z3Z9-mappedCitation-12855688 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12855688 |
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| http://purl.uniprot.org/uniprot/#_F2Z4A5-mappedCitation-12855688 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12855688 |
| http://purl.uniprot.org/uniprot/#_Q3UHZ0-mappedCitation-12855688 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12855688 |