| http://purl.uniprot.org/citations/12859951 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12859951 | http://www.w3.org/2000/01/rdf-schema#comment | "S100A6 (calcyclin) is an acidic calcium binding protein with two EF-hand motifs and overexpressed in several tumors including intrahepatic carcinoma. TNFalpha, a strong NF-kappaB activator required for hepatocyte proliferation during liver regeneration, triggered the expression of S100A6 mRNA in human hepatoblastoma cell line HepG2. Transient expression of NF-kappaB (p65) increased S100A6 promoter activity and expression of inhibitor of NF-kappaB (IkappaBalpha) decreased TNFalpha-induced S100A6 promoter activity. To confirm the involvement of NF-kappaB in S100A6 promoter activation, we analyzed serially deleted promoter constructs of the S100A6 gene by luciferase reporter assay and found a NF-kappaB-responsive DNA fragment at the position between -584 and -361. Electrophoretic mobility shift assays showed that TNFalpha induced p65 binding to a potential NF-kappaB binding site at -460/-451. Furthermore, treatment of cells with CAPE (caffeic acid phenethyl ester), a specific NF-kappaB (p65) inhibitor, decreased NF-kappaB binding and promoter activity. These results suggest that NF-kappaB transcription factor contributes to the activation of S100A6 gene expression in response to TNFalpha in HepG2 cells."xsd:string |
| http://purl.uniprot.org/citations/12859951 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0006-291x(03)01199-9"xsd:string |
| http://purl.uniprot.org/citations/12859951 | http://purl.uniprot.org/core/author | "Kim J.H."xsd:string |
| http://purl.uniprot.org/citations/12859951 | http://purl.uniprot.org/core/author | "Kim J.W."xsd:string |
| http://purl.uniprot.org/citations/12859951 | http://purl.uniprot.org/core/author | "Lee Y."xsd:string |
| http://purl.uniprot.org/citations/12859951 | http://purl.uniprot.org/core/author | "Yoon S.Y."xsd:string |
| http://purl.uniprot.org/citations/12859951 | http://purl.uniprot.org/core/author | "Choe I.S."xsd:string |
| http://purl.uniprot.org/citations/12859951 | http://purl.uniprot.org/core/author | "Joo J.H."xsd:string |
| http://purl.uniprot.org/citations/12859951 | http://purl.uniprot.org/core/author | "Paik S.G."xsd:string |
| http://purl.uniprot.org/citations/12859951 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12859951 | http://purl.uniprot.org/core/name | "Biochem Biophys Res Commun"xsd:string |
| http://purl.uniprot.org/citations/12859951 | http://purl.uniprot.org/core/pages | "274-280"xsd:string |
| http://purl.uniprot.org/citations/12859951 | http://purl.uniprot.org/core/title | "Involvement of NF-kappaB in the regulation of S100A6 gene expression in human hepatoblastoma cell line HepG2."xsd:string |
| http://purl.uniprot.org/citations/12859951 | http://purl.uniprot.org/core/volume | "307"xsd:string |
| http://purl.uniprot.org/citations/12859951 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12859951 |
| http://purl.uniprot.org/citations/12859951 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12859951 |
| http://purl.uniprot.org/uniprot/#_B2R577-mappedCitation-12859951 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12859951 |
| http://purl.uniprot.org/uniprot/#_P06703-mappedCitation-12859951 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12859951 |
| http://purl.uniprot.org/uniprot/B2R577 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12859951 |
| http://purl.uniprot.org/uniprot/P06703 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12859951 |