| http://purl.uniprot.org/citations/12861011 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12861011 | http://www.w3.org/2000/01/rdf-schema#comment | "Melanosomes containing melanin pigments are transported from the cell body of melanocytes to the tips of their dendrites by a combination of microtubule- and actin-dependent machinery. Three proteins, Rab27A, myosin Va, and Slac2-a/melanophilin (a linker protein between Rab27A and myosin Va), are known to be essential for proper actin-based melanosome transport in melanocytes. Although Slac2-a directly interacts with Rab27A and myosin Va via its N-terminal region (amino acids 1 to 146) and the middle region (amino acids 241 to 405), respectively, the functional importance of the putative actin-binding domain of the Slac2-a C terminus (amino acids 401 to 590) in melanosome transport has never been elucidated. In this study we showed that formation of a tripartite protein complex between Rab27A, Slac2-a, and myosin Va alone is insufficient for peripheral distribution of melanosomes in melanocytes and that the C-terminal actin-binding domain of Slac2-a is also required for proper melanosome transport. When a Slac2-a deletion mutant (DeltaABD) or point mutant (KA) that lacks actin-binding ability was expressed in melanocytes, the Slac2-a mutants induced melanosome accumulation in the perinuclear region, possibly by a dominant negative effect, the same as the Rab27A-binding-defective mutant of Slac2-a or the myosin Va-binding-defective mutant. Our findings indicate that Slac2-a organizes actin-based melanosome transport in cooperation with Rab27A, myosin Va, and actin."xsd:string |
| http://purl.uniprot.org/citations/12861011 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.23.15.5245-5255.2003"xsd:string |
| http://purl.uniprot.org/citations/12861011 | http://purl.uniprot.org/core/author | "Fukuda M."xsd:string |
| http://purl.uniprot.org/citations/12861011 | http://purl.uniprot.org/core/author | "Ariga H."xsd:string |
| http://purl.uniprot.org/citations/12861011 | http://purl.uniprot.org/core/author | "Kuroda T.S."xsd:string |
| http://purl.uniprot.org/citations/12861011 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12861011 | http://purl.uniprot.org/core/name | "Mol Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/12861011 | http://purl.uniprot.org/core/pages | "5245-5255"xsd:string |
| http://purl.uniprot.org/citations/12861011 | http://purl.uniprot.org/core/title | "The actin-binding domain of Slac2-a/melanophilin is required for melanosome distribution in melanocytes."xsd:string |
| http://purl.uniprot.org/citations/12861011 | http://purl.uniprot.org/core/volume | "23"xsd:string |
| http://purl.uniprot.org/citations/12861011 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12861011 |
| http://purl.uniprot.org/citations/12861011 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12861011 |
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| http://purl.uniprot.org/uniprot/A0A0R4J075 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12861011 |
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| http://purl.uniprot.org/uniprot/B9EKA5 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12861011 |