| http://purl.uniprot.org/citations/12872131 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12872131 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12872131 | http://www.w3.org/2000/01/rdf-schema#comment | "There is a growing need for techniques that can identify and characterize protein modifications on a large or global scale. We report here a proteomics approach to enrich, recover, and identify ubiquitin conjugates from Saccharomyces cerevisiae lysate. Ubiquitin conjugates from a strain expressing 6xHis-tagged ubiquitin were isolated, proteolyzed with trypsin and analyzed by multidimensional liquid chromatography coupled with tandem mass spectrometry (LC/LC-MS/MS) for amino acid sequence determination. We identified 1,075 proteins from the sample. In addition, we detected 110 precise ubiquitination sites present in 72 ubiquitin-protein conjugates. Finally, ubiquitin itself was found to be modified at seven lysine residues providing evidence for unexpected diversity in polyubiquitin chain topology in vivo. The methodology described here provides a general tool for the large-scale analysis and characterization of protein ubiquitination."xsd:string |
| http://purl.uniprot.org/citations/12872131 | http://purl.org/dc/terms/identifier | "doi:10.1038/nbt849"xsd:string |
| http://purl.uniprot.org/citations/12872131 | http://purl.org/dc/terms/identifier | "doi:10.1038/nbt849"xsd:string |
| http://purl.uniprot.org/citations/12872131 | http://purl.uniprot.org/core/author | "Finley D."xsd:string |
| http://purl.uniprot.org/citations/12872131 | http://purl.uniprot.org/core/author | "Finley D."xsd:string |
| http://purl.uniprot.org/citations/12872131 | http://purl.uniprot.org/core/author | "Elias J.E."xsd:string |
| http://purl.uniprot.org/citations/12872131 | http://purl.uniprot.org/core/author | "Elias J.E."xsd:string |
| http://purl.uniprot.org/citations/12872131 | http://purl.uniprot.org/core/author | "Gygi S.P."xsd:string |
| http://purl.uniprot.org/citations/12872131 | http://purl.uniprot.org/core/author | "Gygi S.P."xsd:string |
| http://purl.uniprot.org/citations/12872131 | http://purl.uniprot.org/core/author | "Peng J."xsd:string |
| http://purl.uniprot.org/citations/12872131 | http://purl.uniprot.org/core/author | "Peng J."xsd:string |
| http://purl.uniprot.org/citations/12872131 | http://purl.uniprot.org/core/author | "Schwartz D."xsd:string |
| http://purl.uniprot.org/citations/12872131 | http://purl.uniprot.org/core/author | "Schwartz D."xsd:string |
| http://purl.uniprot.org/citations/12872131 | http://purl.uniprot.org/core/author | "Cheng D."xsd:string |
| http://purl.uniprot.org/citations/12872131 | http://purl.uniprot.org/core/author | "Cheng D."xsd:string |
| http://purl.uniprot.org/citations/12872131 | http://purl.uniprot.org/core/author | "Roelofs J."xsd:string |
| http://purl.uniprot.org/citations/12872131 | http://purl.uniprot.org/core/author | "Roelofs J."xsd:string |
| http://purl.uniprot.org/citations/12872131 | http://purl.uniprot.org/core/author | "Marsischky G."xsd:string |
| http://purl.uniprot.org/citations/12872131 | http://purl.uniprot.org/core/author | "Marsischky G."xsd:string |
| http://purl.uniprot.org/citations/12872131 | http://purl.uniprot.org/core/author | "Thoreen C.C."xsd:string |
| http://purl.uniprot.org/citations/12872131 | http://purl.uniprot.org/core/author | "Thoreen C.C."xsd:string |
| http://purl.uniprot.org/citations/12872131 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12872131 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |