| http://purl.uniprot.org/citations/12874278 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12874278 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12874278 | http://www.w3.org/2000/01/rdf-schema#comment | "Wnt signaling is essential during development while deregulation of this pathway frequently leads to the formation of various tumors including colorectal carcinomas. A key component of the pathway is beta-catenin that, in association with TCF-4, directly regulates the expression of Wnt-responsive genes. To identify novel binding partners of beta-catenin that may control its transcriptional activity, we performed a mammalian two-hybrid screen and isolated the Tax-interacting protein (TIP-1). The in vivo complex formation between beta-catenin and TIP-1 was verified by coimmunoprecipitation, and a direct physical association was revealed by glutathione S-transferase pull-down experiments in vitro. By using a panel of deletion mutants of both proteins, we demonstrate that the interaction is mediated by the PDZ (PSD-95/DLG/ZO-1 homology) domain of TIP-1 and requires primarily the last four amino acids of beta-catenin. TIP-1 overexpression resulted in a dose-dependent decrease in the transcriptional activity of beta-catenin when tested on the TOP/FOPFLASH reporter system. Conversely, siRNA-mediated knock-down of endogenous TIP-1 slightly increased endogenous beta-catenin transactivation function. Moreover, we show that overexpression of TIP-1 reduced the proliferation and anchorage-independent growth of colorectal cancer cells. These data suggest that TIP-1 may represent a novel regulatory element in the Wnt/beta-catenin signaling pathway."xsd:string |
| http://purl.uniprot.org/citations/12874278 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m306324200"xsd:string |
| http://purl.uniprot.org/citations/12874278 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m306324200"xsd:string |
| http://purl.uniprot.org/citations/12874278 | http://purl.uniprot.org/core/author | "Hayashizaki Y."xsd:string |
| http://purl.uniprot.org/citations/12874278 | http://purl.uniprot.org/core/author | "Hayashizaki Y."xsd:string |
| http://purl.uniprot.org/citations/12874278 | http://purl.uniprot.org/core/author | "Kai C."xsd:string |
| http://purl.uniprot.org/citations/12874278 | http://purl.uniprot.org/core/author | "Kai C."xsd:string |
| http://purl.uniprot.org/citations/12874278 | http://purl.uniprot.org/core/author | "Kanamori M."xsd:string |
| http://purl.uniprot.org/citations/12874278 | http://purl.uniprot.org/core/author | "Kanamori M."xsd:string |
| http://purl.uniprot.org/citations/12874278 | http://purl.uniprot.org/core/author | "Suzuki H."xsd:string |
| http://purl.uniprot.org/citations/12874278 | http://purl.uniprot.org/core/author | "Suzuki H."xsd:string |
| http://purl.uniprot.org/citations/12874278 | http://purl.uniprot.org/core/author | "Schneider C."xsd:string |
| http://purl.uniprot.org/citations/12874278 | http://purl.uniprot.org/core/author | "Schneider C."xsd:string |
| http://purl.uniprot.org/citations/12874278 | http://purl.uniprot.org/core/author | "Benetti R."xsd:string |
| http://purl.uniprot.org/citations/12874278 | http://purl.uniprot.org/core/author | "Benetti R."xsd:string |
| http://purl.uniprot.org/citations/12874278 | http://purl.uniprot.org/core/author | "Marzinotto S."xsd:string |
| http://purl.uniprot.org/citations/12874278 | http://purl.uniprot.org/core/author | "Marzinotto S."xsd:string |
| http://purl.uniprot.org/citations/12874278 | http://purl.uniprot.org/core/author | "Sandy P."xsd:string |
| http://purl.uniprot.org/citations/12874278 | http://purl.uniprot.org/core/author | "Sandy P."xsd:string |
| http://purl.uniprot.org/citations/12874278 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12874278 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12874278 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12874278 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |