| http://purl.uniprot.org/citations/12881429 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12881429 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12881429 | http://www.w3.org/2000/01/rdf-schema#comment | "Aberrant mRNAs containing premature termination codons (PTC-mRNAs) are degraded by a conserved surveillance system, referred to as the nonsense-mediated decay (NMD) pathway. Although NMD is reported to operate on the decapping and 5'-to-3' exonucleolytic decay of PTC-mRNAs without affecting deadenylation, a role for an opposite 3'-to-5' decay pathway remains largely unexplored. In this study, we have characterized the 3'-to-5' directed mRNA degradation in the yeast NMD pathway. PTC-mRNAs are stabilized in yeast cells lacking the components of 3'-to-5' mRNA-decay machinery. The 3'-to-5' directed degradation of PTC-mRNAs proceeds more rapidly than that of the PTC-free transcript, in a manner dependent on the cytoplasmic exosome and Upf proteins. Moreover, Upf1p, but not Upf2p, interacts physically with an N-terminal domain of Ski7p, although the interaction requires Upf2p. The efficiency of 3'-to-5' directed degradation of PTC-mRNAs is impaired by overexpression of Ski7p N-domain fragments that contain a sequence of the Upf1p-interaction region. These data suggest that the activation of 3'-to-5' directed NMD is mediated through the interaction between Upf1p and the Ski7p N domain."xsd:string |
| http://purl.uniprot.org/citations/12881429 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/cdg374"xsd:string |
| http://purl.uniprot.org/citations/12881429 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/cdg374"xsd:string |
| http://purl.uniprot.org/citations/12881429 | http://purl.uniprot.org/core/author | "Takahashi S."xsd:string |
| http://purl.uniprot.org/citations/12881429 | http://purl.uniprot.org/core/author | "Takahashi S."xsd:string |
| http://purl.uniprot.org/citations/12881429 | http://purl.uniprot.org/core/author | "Sakuno T."xsd:string |
| http://purl.uniprot.org/citations/12881429 | http://purl.uniprot.org/core/author | "Sakuno T."xsd:string |
| http://purl.uniprot.org/citations/12881429 | http://purl.uniprot.org/core/author | "Araki Y."xsd:string |
| http://purl.uniprot.org/citations/12881429 | http://purl.uniprot.org/core/author | "Araki Y."xsd:string |
| http://purl.uniprot.org/citations/12881429 | http://purl.uniprot.org/core/author | "Katada T."xsd:string |
| http://purl.uniprot.org/citations/12881429 | http://purl.uniprot.org/core/author | "Katada T."xsd:string |
| http://purl.uniprot.org/citations/12881429 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12881429 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12881429 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/12881429 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/12881429 | http://purl.uniprot.org/core/pages | "3951-3959"xsd:string |
| http://purl.uniprot.org/citations/12881429 | http://purl.uniprot.org/core/pages | "3951-3959"xsd:string |
| http://purl.uniprot.org/citations/12881429 | http://purl.uniprot.org/core/title | "Interaction between Ski7p and Upf1p is required for nonsense-mediated 3'-to-5' mRNA decay in yeast."xsd:string |
| http://purl.uniprot.org/citations/12881429 | http://purl.uniprot.org/core/title | "Interaction between Ski7p and Upf1p is required for nonsense-mediated 3'-to-5' mRNA decay in yeast."xsd:string |
| http://purl.uniprot.org/citations/12881429 | http://purl.uniprot.org/core/volume | "22"xsd:string |
| http://purl.uniprot.org/citations/12881429 | http://purl.uniprot.org/core/volume | "22"xsd:string |
| http://purl.uniprot.org/citations/12881429 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12881429 |
| http://purl.uniprot.org/citations/12881429 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12881429 |