| http://purl.uniprot.org/citations/12881487 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12881487 | http://www.w3.org/2000/01/rdf-schema#comment | "Dynamic regulation of ion channels is critical for maintaining fluid balance in epithelial tissues. Cystic fibrosis, a genetic disease characterized by impaired fluid transport in epithelial tissues, is caused by dysfunctional cystic fibrosis transmembrane conductance regulator (CFTR) Cl-channel activity. Recent studies have shown that binding of PSD-95/Dlg/ZO-1 (PDZ) domain proteins to CFTR is important for retaining it at the apical membrane and for regulating its channel activity. Here, we describe a phosphorylation mechanism that regulates CFTR channel activity, which is mediated by PDZ domains. The Na+/H+ exchanger regulatory factor (NHERF) binds to CFTR and increases its open probability (Po). Protein kinase C disrupts the stimulatory effect of NHERF on CFTR channel Po. Phosphorylation by PKC of Ser-162 in the PDZ2 domain of NHERF is critical for this functional effect. Furthermore, a mutation in PDZ2 that mimics phosphorylation decreases CFTR binding and disrupts the ability of NHERF PDZ1-2 to stimulate CFTR channel Po. Our results identify a role for PKC and suggest that phosphorylation of NHERF PDZ2 domain may be an important mechanism for regulating CFTR channel activity."xsd:string |
| http://purl.uniprot.org/citations/12881487 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1633250100"xsd:string |
| http://purl.uniprot.org/citations/12881487 | http://purl.uniprot.org/core/author | "Foskett J.K."xsd:string |
| http://purl.uniprot.org/citations/12881487 | http://purl.uniprot.org/core/author | "Raghuram V."xsd:string |
| http://purl.uniprot.org/citations/12881487 | http://purl.uniprot.org/core/author | "Hormuth H."xsd:string |
| http://purl.uniprot.org/citations/12881487 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12881487 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/12881487 | http://purl.uniprot.org/core/pages | "9620-9625"xsd:string |
| http://purl.uniprot.org/citations/12881487 | http://purl.uniprot.org/core/title | "A kinase-regulated mechanism controls CFTR channel gating by disrupting bivalent PDZ domain interactions."xsd:string |
| http://purl.uniprot.org/citations/12881487 | http://purl.uniprot.org/core/volume | "100"xsd:string |
| http://purl.uniprot.org/citations/12881487 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12881487 |
| http://purl.uniprot.org/citations/12881487 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12881487 |
| http://purl.uniprot.org/uniprot/O14745#attribution-5FE9025A19849B083F20BC73156C0490 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/12881487 |
| http://purl.uniprot.org/uniprot/#_A0A089NFM8-mappedCitation-12881487 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12881487 |
| http://purl.uniprot.org/uniprot/#_A0A089PPW6-mappedCitation-12881487 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12881487 |
| http://purl.uniprot.org/uniprot/#_A0A088T1A1-mappedCitation-12881487 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12881487 |
| http://purl.uniprot.org/uniprot/#_A0A096ZUB9-mappedCitation-12881487 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12881487 |
| http://purl.uniprot.org/uniprot/#_A0A096ZUC0-mappedCitation-12881487 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12881487 |
| http://purl.uniprot.org/uniprot/#_A0A096ZUC4-mappedCitation-12881487 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12881487 |
| http://purl.uniprot.org/uniprot/#_A0A096ZUH4-mappedCitation-12881487 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12881487 |
| http://purl.uniprot.org/uniprot/#_A0A096ZUB3-mappedCitation-12881487 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12881487 |
| http://purl.uniprot.org/uniprot/#_A0A096ZUB7-mappedCitation-12881487 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12881487 |
| http://purl.uniprot.org/uniprot/#_A0A096ZUB8-mappedCitation-12881487 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12881487 |
| http://purl.uniprot.org/uniprot/#_A0A1B0YU32-mappedCitation-12881487 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12881487 |